Macrophages and dendritic cells constitute a major subpopulation of cells in the mouse dermis
Macrophages and dendritic cells constitute a major subpopulation of cells in the mouse dermis
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DOI：
10.1111/j.0022-202x.2004.23427.x
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发表时间：
2004-11-01
影响因子：
6.5
通讯作者：
Leenen, PJM
中科院分区：
文献类型：
--
作者：
Dupasquier, M;Stoitzner, P;Leenen, PJM
与环境密切接触的组织中的巨噬细胞和树突状细胞（DC）在宿主防御中具有至关重要的作用，因此存在相当大的数量。因此，令人惊讶的是，真皮的单核吞噬细胞群体很少以定量的方式进行研究。在这项研究中，我们研究了小鼠真皮皮肤免疫表型和相关的观察到的细胞数量的有核细胞的总数。这些分析表明，大约70%的真皮细胞代表CD 45（+）白细胞。这些细胞中的绝大多数（约占总数的60%）表达单核吞噬细胞标志物mMGL（ER-MP23）、F4/80和CD 11b。此外，这些细胞显示出贪婪的吞噬能力，因此被鉴定为真皮巨噬细胞。不同的亚群可以使用标记物如唾液酸粘附素、ER-HR 3和mSIGN-R1（ER-TR 9）来定义。有趣的是，MHCII类表达显着不同的真皮细胞从耳朵与背部皮肤。此外，我们还发现了少量的真皮DC和迁移的朗格汉斯细胞（共约占总数的10%）。总之，我们的研究结果表明，单核吞噬细胞群体形成的真皮细胞的大部分，因此已被明显低估了。
Macrophages and dendritic cells (DC) in tissues with close contact to the environment are of essential importance in host defense and are therefore present in sizeable numbers. Therefore, it is surprising that mononuclear phagocyte populations of the dermis have rarely been investigated in a quantitative manner. In this study, we examined mouse dermal skin immunophenotypically and related the observed numbers of observed cells to the total number of nucleated cells. These analyses show that about 70% of all dermal cells represent CD45(+) leukocytes. The vast majority of these cells (similar to60% of total) expresses the mononuclear phagocyte markers mMGL (ER-MP23), F4/80 and CD11b. In addition, these cells show avid phagocytic capacity and thus are identified as dermal macrophages. Different subpopulations can be defined using markers such as sialoadhesin, ER-HR3 and mSIGN-R1 (ER-TR9). Interestingly, MHC class II expression differs significantly between dermal cells from ear versus back skin. Moreover, we have identified small populations of dermal DC and migrating Langerhans cells (together similar to10% of total). In summary, our findings show that mononuclear phagocyte populations form the majority of dermal cells and thus have been clearly underestimated so far.