Hydrogen peroxide modulates whole cell Ca2+ currents through L-type channels in cultured rat dentate granule cells
Hydrogen peroxide modulates whole cell Ca2+ currents through L-type channels in cultured rat dentate granule cells
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DOI：
10.1016/j.neulet.2003.11.012
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发表时间：
2004-02-06
影响因子：
2.5
通讯作者：
Ito, Y
中科院分区：
文献类型：
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作者：
Akaishi, T;Nakazawa, K;Ito, Y
使用全细胞膜片钳技术检查了培养的齿状颗粒细胞中过氧化氢（一种膜渗透形式的活性氧）对电压门控 Ca2+ 通道的修饰。用过氧化氢（1 和 10 μM）预处理 2 小时增强了 Ca2+ 电流而不影响其电压依赖性。这种增强作用被 1 mM 谷胱甘肽（一种抗氧化剂）和 2 mM 硝苯地平（一种 L 型 Ca2+ 通道阻滞剂）完全抵消。相反，用 10 μg/ml 衣霉素（一种内质网应激源）预处理并不能模拟 Ca2+ 电流的增强。这些结果表明，过氧化氢诱导的氧化应激选择性调节 L 型 Ca2+ 通道的活性。 (C) 2003 Elsevier Ireland Ltd. 保留所有权利。
Modification of voltage-gated Ca2+ channels by hydrogen peroxide, a membrane-permeable form of reactive oxygen species, in cultured dentate granule cells was examined using the whole cell patch clamp technique. Pretreatment with hydrogen peroxide (1 and 10 muM) for 2 h enhanced the Ca2+ current without affecting its voltage dependence. The enhancement was completely cancelled by 1 mM glutathione, an antioxidant, and 2 muM nifedipine, an L-type Ca2+ channel blocker. In contrast, the enhancement of the Ca2+ current was not mimicked by pretreatment with 10 mug/ml tunicamycin, an endoplasmic reticulum stressor. These results suggest that oxidative stress induced by hydrogen peroxide selectively regulates the activity of L-type Ca2+ channels. (C) 2003 Elsevier Ireland Ltd. All rights reserved.