Granulocyte-macrophage colony-stimulating factor promotes human blastocyst development in vitro
Granulocyte-macrophage colony-stimulating factor promotes human blastocyst development in vitro
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DOI：
10.1093/humrep/14.12.3069
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发表时间：
1999-12-01
影响因子：
6.1
通讯作者：
Robertson, SA
中科院分区：
文献类型：
--
作者：
Sjöblom, C;Wikland, M;Robertson, SA
细胞因子粒细胞-巨噬细胞集落刺激因子（GM-CSF）在雌性生殖道中合成，并与啮齿动物和家畜种属的植入前胚胎的生长和发育有关。为了检测GM-CSF对体外人胚胎发育的影响，将多余的冷冻2-4细胞胚胎在补充有2ngl ml重组人GM-CSF的培养基中培养。细胞因子的添加使发育到囊胚阶段的胚胎比例从30%增加到76%。通过孵化和附着到细胞外基质包被的培养皿来评估这些囊胚的发育能力，GM-CSF也改善了这些囊胚的发育能力。形成囊胚总数的50%所需的培养时间减少了14小时，并且在GM-CSF中生长的囊胚：发现含有类似于35%以上的细胞，主要是由于内部细胞团的大小增加。GM-CSF的有益作用在两种连续培养基系统（IVF-50/S2和G1.2/G2.2）中的每一种中发挥，并且与所使用的重组细胞因子的制剂无关。这些数据表明，GM-CSF可能有生理作用，促进发展：人类胚胎，因为它穿越生殖道在体内，并建议，除了这种细胞因子的胚胎培养基中可能会提高产量的体外受精程序的发育能力的囊胚。
The cytokine granulocyte-macrophage colony-stimulating factor (GM-CSF) is synthesized in the female reproductive tract and has been implicated in the growth and development of the preimplantation embryo in rodent and livestock species. To examine the effect of GM-CSF on human embryo development in vitro, surplus frozen 2-4-cell embryos were cultured in media supplemented with 2 ngl mi recombinant human GM-CSF. The addition of cytokine increased the proportion of embryos that developed to the blastocyst stage from 30 to 76%, The developmental competence of these blastocysts, as assessed by hatching and attachment to extracellular matrix-coated culture dishes, was also improved by GM-CSF, The period in culture required for 50% of the total number of blastocysts to form was reduced by 14 h, and blastocysts grown in GM-CSF: were found to contain similar to 35% more cells, due primarily to an increase in the size of the inner cell mass. The beneficial effect of GM-CSF was exerted in each of two sequential media systems (IVF-50/S2 and G1.2/G2.2) and was independent of the formulation of recombinant cytokine that was used. These data indicate that GM-CSF may have a physiological role in promoting the development of the: human embryo as it traverses the reproductive tract in vivo, and suggest that addition of this cytokine to embryo culture media may improve the yield of implantation-competent blastocysts in human in-vitro fertilization programmes.