EPIDERMAL GROWTH-FACTOR RAPIDLY STIMULATES PROLACTIN GENE-TRANSCRIPTION
EPIDERMAL GROWTH-FACTOR RAPIDLY STIMULATES PROLACTIN GENE-TRANSCRIPTION
复制标题
DOI：
10.1038/300192a0
复制
发表时间：
1982-01-01
期刊：
影响因子：
64.8
通讯作者：
ROSENFELD, MG
中科院分区：
文献类型：
--
作者：
MURDOCH, GH;POTTER, E;ROSENFELD, MG
表皮生长因子（EGF）最初被认为是多种细胞类型的生长因子1,2，随后被证明影响许多细胞和分子过程3,4，其中许多可能被认为是多效生长反应的一部分（增强葡萄糖、尿苷和氨基酸的摄取，并刺激蛋白质、RNA和DNA的合成）。对EGF的早期反应，如钠通量的增加和酪氨酸磷酸化的刺激，被认为介导了EGF的部分或全部作用。在许多组织中，EGF已被证明可以增加特定蛋白的合成7 - 10。我们研究了EGF对gh4大鼠垂体细胞系催乳素合成的影响，以进一步了解EGF作用于细胞功能的机制。在gh4细胞中添加EGF会导致催乳素合成的三到六倍刺激，并部分抑制细胞生长7,8。在本报告中，我们证明了催乳素合成的增加似乎是EGF快速刺激催乳素基因转录的结果。这很容易让人猜测，对特定基因的早期转录作用，如本文报道的gh4细胞中催乳素基因的情况，可能介导EGF在其作为生长因子的细胞中对细胞周期调节的部分或全部后期作用。
Epidermal growth factor (EGF) was originally characterized as a growth factor for various cell types1,2and was subsequently shown to affect a number of cellular and molecular processes3,4, of which many might be considered as a part of the pleiotropic growth response (enhanced uptake of glucose, uridine and amino acids and stimulated synthesis of protein, RNA and DNA). Very early responses to EGF, such as increased sodium fluxes5and stimulation of tyrosine phosphorylation6, have been proposed to mediate some or all of EGF's effects. In a number of tissues, EGF has been shown to increase the synthesis of specific proteins7–10. We have investigated the effects of EGF on prolactin synthesis in the GH4rat pituitary cell line to gain further insight into the mechanism of EGF's actions on cellular functions. Addition of EGF to GH4cells results in a three- to sixfold stimulation of prolactin synthesis, as well as a partial inhibition of cell growth7,8. In this report, we demonstrate that the increased prolactin synthesis appears to be the result of a rapid stimulation of prolactin gene transcription by EGF. It is tempting to speculate that very early transcriptional effects on specific genes, such as reported here in the case of the prolactin gene in GH4cells, may mediate some or all of the later effects of EGF on cell cycle regulation in those cells for which it serves as a growth factor.