Dietary regulation of sucrase-isomaltase gene expression in rat jejunum.
Dietary regulation of sucrase-isomaltase gene expression in rat jejunum.
复制标题
大鼠空肠中蔗糖酶-异麦芽糖酶基因表达的饮食调节。
DOI：
10.1016/0304-4165(94)00143-l
复制
发表时间：
1995
期刊：
影响因子：
--
通讯作者：
Sachiko Takase
中科院分区：
文献类型：
--
作者：
H. Yasutake;Toshinao Goda;Sachiko Takase
我们先前已经证明，脂肪和碳水化合物的摄入影响大鼠空肠蔗糖酶异麦芽糖酶(S-I)的活性和免疫反应量。为了探讨蔗糖酶和异麦芽糖酶活性的改变是否伴随着蔗糖酶异麦芽糖酶mRNA水平的变化，7周龄大鼠分别饲喂高长链三酰甘油(73能%玉米油)、高中链三酰甘油(MCT)饲料(66能%玉米油，7能%玉米油)和高碳水化合物饲料(70能%玉米淀粉)。Northern印迹分析显示，饲喂高MCT饲料的大鼠空肠组织中存在丰富的S-I基因表达，与饲喂高碳水化合物饲料的大鼠相似。在12h内，强迫饲喂高蔗糖饲料(40%蔗糖)可同时引起S-imRNA和钠/d-葡萄糖共转运体(SGLT1mRNA)水平的升高，同时也引起S-imRNA和SGLT1mRNA水平的升高。然而，强行饲喂含有α-甲基葡萄糖苷的饲料并没有增加S-imRNA和SGLT1mRNA的水平，但蔗糖酶活性却通过饲喂α-甲基葡萄糖苷而提高。这些结果表明，不仅碳水化合物摄入量而且MCT摄入量可能通过碳水化合物和MCT的共同代谢产物(S)影响空肠S-imRNA和SGLT1mRNA水平，碳水化合物可能通过调节蔗糖酶-异麦芽糖酶复合体的翻译和/或翻译后修饰而在提高蔗糖酶活性方面发挥另一作用。
We have previously demonstrated that intake of fat as well as carbohydrate affects the activity and immunoreactive amount of sucrase-isomaltase (S-I) in rat jejunum. To examine whether diet-related changes in sucrase and isomaltase activities are accompanied by the variations of sucrase-isomaltase mRNA levels, 7-week-old rats were fed either a high-long-chain triacylglycerols diet (73 energy% as corn oil), a high-medium-chain triacylglycerols (MCT) diet (66 energy% as MCT, 7 energy% as corn oil) or a high-carbohydrate diet (70 energy% as corn starch) for 7 days. Northern blot analysis revealed that S-I mRNA levels were abundant in the jejunum of rats fed the high-MCT diet; the levels were similar to those in the rats fed the high-carbohydrate diet. Force-feeding a high-sucrose diet (40 energy% as sucrose) brought about a parallel rise in both S-I mRNA and sodium/d-glucose cotransporter (SGLT1) mRNA levels within 12 h. Force-feeding the high-MCT diet also produced an elevation of S-I mRNA and SGLT1 mRNA. However, force-feeding a diet containing α-methylglucoside, a non-metabolizable but actively transported sugar, did not increase S-I mRNA or SGLT1 mRNA level; sucrase activity was nevertheless elevated by feeding α-methylglucoside diet. These results suggest that not only carbohydrate intake but also MCT intake might influence S-I mRNA and SGLT1 mRNA levels in the jejunum, presumably through common metabolite(s) of carbohydrates and MCT, and that carbohydrate may play another role in enhancement of the sucrase activity through modulation of translation and/or posttranslational modifications of the sucrase-isomaltase complex.
饮食诱导成年大鼠空肠中乳糖酶活性和免疫反应性乳糖酶的增加。
DOI：
10.1042/bj2210261
发表时间：
1984
期刊：
The Biochemical journal
影响因子：
--
作者：
Goda,T;Bustamante,S;Thornburg,W;Koldovský,O
通讯作者：
Koldovský,O