F-SPONDIN - A GENE EXPRESSED AT HIGH-LEVELS IN THE FLOOR PLATE ENCODES A SECRETED PROTEIN THAT PROMOTES NEURAL CELL-ADHESION AND NEURITE EXTENSION
F-SPONDIN - A GENE EXPRESSED AT HIGH-LEVELS IN THE FLOOR PLATE ENCODES A SECRETED PROTEIN THAT PROMOTES NEURAL CELL-ADHESION AND NEURITE EXTENSION
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DOI：
10.1016/0092-8674(92)90121-r
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发表时间：
1992-04-03
期刊：
影响因子：
64.5
通讯作者：
JESSELL, TM
中科院分区：
文献类型：
--
作者：
KLAR, A;BALDASSARE, M;JESSELL, TM
底板是在发育中的脊椎动物神经系统中涉及控制神经细胞模式和轴突生长的细胞群。为了鉴定可能介导底板功能的分子，我们使用消减杂交技术分离底板富集的cDNA克隆。一个这样的克隆编码一种新的分泌蛋白，F-spondin，其在底板中以高水平表达。该蛋白质的C-末端的一半包含先前在血小板反应蛋白和其他涉及细胞粘附的蛋白质中鉴定的六个重复。F-spondin在轴突第一次延伸时在底板中表达，并且在外周神经中以较低水平表达。重组F-spondin在体外促进脊髓和感觉神经元细胞的附着和神经突起的生长。F-spondin可能有助于脊髓和PNS中轴突的生长和引导。
The floor plate is a cell group implicated in the control of neural cell pattern and axonal growth in the developing vertebrate nervous system. To identify molecules that might mediate the functions of the floor plate, we have used subtractive hybridization techniques to isolate floor plate-enriched cDNA clones. One such clone encodes a novel secreted protein, F-spondin, which is expressed at high levels in the floor plate. The C-terminal half of the protein contains six repeats identified previously in thrombospondin and other proteins implicated in cell adhesion. F-spondin is expressed in the floor plate at the time that axons first extend and at lower levels in the peripheral nerve. Recombinant F-spondin promotes the attachment of spinal cord and sensory neuron cells and the out-growth of neurites in vitro. F-spondin may contribute to the growth and guidance of axons in both the spinal cord and the PNS.