Midline governs axon pathfinding by coordinating expression of two major guidance systems
Midline governs axon pathfinding by coordinating expression of two major guidance systems
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DOI：
10.1101/gad.1774209
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发表时间：
2009-05-15
影响因子：
10.5
通讯作者：
Hirose, Susumu
中科院分区：
文献类型：
--
作者：
Liu, Qing-Xin;Hiramoto, Masaki;Hirose, Susumu
神经网络的形成需要多个轴突引导系统的协同作用。人们对神经元如何协调多个引导基因的表达知之甚少。在这里，我们展示了果蝇 T-box 蛋白 Midline 控制编码两个主要引导系统组件的基因的表达：Frazzled、ROBO 和 Slit。在中线突变体中，所有这些分子的表达均减少，导致严重的轴突引导缺陷，而中线的错误表达则诱导它们的表达。中线存在于这些基因的启动子区域，表明中线直接控制转录。我们建议中线通过协调两个引导系统来控制轴突寻路。
Formation of the neural network requires concerted action of multiple axon guidance systems. How neurons orchestrate expression of multiple guidance genes is poorly understood. Here, we show that Drosophila T-box protein Midline controls expression of genes encoding components of two major guidance systems: Frazzled, ROBO, and Slit. In midline mutant, expression of all these molecules are reduced, resulting in severe axon guidance defects, whereas misexpression of Midline induces their expression. Midline is present on the promoter regions of these genes, indicating that Midline controls transcription directly. We propose that Midline controls axon pathfinding through coordinating the two guidance systems.