ULTRASTRUCTURAL-LOCALIZATION OF GENTAMICIN IN THE COCHLEA
ULTRASTRUCTURAL-LOCALIZATION OF GENTAMICIN IN THE COCHLEA
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DOI：
10.1016/0378-5955(90)90031-j
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发表时间：
1990-12-01
期刊：
影响因子：
2.8
通讯作者：
VELDMAN, JE
中科院分区：
文献类型：
--
作者：
DEGROOT, JCMJ;MEEUWSEN, F;VELDMAN, JE
采用免疫细胞化学方法研究了庆大霉素在耳蜗中的超微结构分布。 特异性标记仅限于柯蒂氏器，特别是外毛细胞和内毛细胞、Deiters 细胞、Hensen 细胞和基底膜的鼓室层细胞。 其他耳蜗组织没有显示任何标记。 在亚细胞水平，庆大霉素存在于溶酶体、多泡体以及小管和囊泡中。 提出了一个模型，其中假设庆大霉素被内吞小泡内化并转移到溶酶体区室以及内质网和高尔基复合体。
The ultrastructural distribution of gentamicin in the cochlea was investigated immunocytochemically. Specific labeling was restricted to the organ of Corti, in particular to the outer and inner hair cells, the Deiters' cells, Hensen's cells and the tympanic layer cells of the basilar membrane. Other cochlear tissues did not demonstrate any labeling. At the subcellular level, gentamicin was found in lysosomes, multivesicular bodies and small tubules and vesicles. A model is proposed in which it is hypothesized that gentamicin is internalized by endocytotic vesicles and is transferred to the lysosomal compartment as well as to the endoplasmic reticulum and Golgi complex.