Gas chromatography of methyl glycosides as their trimethylsilyl ethers
Gas chromatography of methyl glycosides as their trimethylsilyl ethers
复制标题
甲基糖苷三甲基硅醚的气相色谱分析
DOI：
10.1016/s0021-9673(00)89212-2
复制
发表时间：
1977
影响因子：
4.1
通讯作者：
C. Todd
中科院分区：
文献类型：
--
作者：
D. Pritchard;C. Todd
Gas chromatographic procedures for the analysis of carbohydrates in glyco-proteins frequently include methanolysis and re-N-acetylation steps. The usual methanolysis conditions were found to result in almost complete conversion of the methanolic HCl into methyl chloride. A neutral solution of methyl chloride in methanol was found capable of releasing fucose, sialic acid, and a portion of the galactose froma1-acid glycoprotein.During the re-N-acetylation of the amino sugars O-acetylation of the alditols commonly employed as internal standards occurs. Addition of the standards after the re-N-acetylation step avoided the problem. The complete procedure for the determination of both neutral and amino sugars has been simplified to involve only one transfer step prior to final gas chromatography.
