Establishment of oral anaerobes during the first year of life
Establishment of oral anaerobes during the first year of life
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DOI：
10.1177/00220345990780100801
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发表时间：
1999-10-01
影响因子：
7.6
通讯作者：
Jousimies-Somer, H
中科院分区：
文献类型：
--
作者：
Könönen, E;Kanervo, A;Jousimies-Somer, H
厌氧菌是口腔细菌群落的重要组成部分。尽管婴儿初次定植的时间和种类是形成进一步定植的基础，因此非常重要，但口腔厌氧菌群随年龄的发展仍未得到充分了解。在本研究中，对44名2、6和12个月大的健康高加索婴儿的口腔厌氧菌定植时间和顺序进行了纵向研究。未刺激唾液标本在非选择性布鲁氏菌血琼脂和几种选择性培养基上定量培养，分离厌氧微生物。在两个月大的婴儿中，最常见的厌氧菌是韦洛氏菌。产黑素普氏杆菌群也是早期定殖种的代表，其出现频率在第一年显著增加，而中间普氏氏菌群生物似乎是较晚的定殖者。在2个月大的受试者中偶尔会发现核梭杆菌、无色素普雷沃特氏菌和核卟啉单胞菌，但在一岁大的受试者中经常会发现。核杆菌是一岁婴儿中最常见的严格厌氧菌；偶尔也会发现其他梭菌。兼性/微好氧腐蚀杆和吞噬藻属的出现频率。在接近第一年年底的时候开始增加。除了常见的兼性/微嗜氧放线菌外，其他厌氧革兰氏阳性菌仅在这些婴儿中偶尔存在。一旦确定，早期定居的物种往往会在口腔中持续存在。我们的纵向研究表明，在生命的第一年，几种厌氧菌的出现频率稳步上升。
Anaerobic species constitute a significant part of the bacterial community of the mouth. Although the time and species involved in the primary colonization of infants are of great importance by forming the basis for further colonization, the development of the oral anaerobic microflora with age is still inadequately understood. In the present study, time and succession of colonization of oral anaerobes were longitudinally examined in 44 healthy Caucasian infants at 2, 6, and 12 months of age. Unstimulated saliva samples were quantitatively cultured on non-selective Brucella blood agar and several selective media for the isolation of anaerobic micro-organisms. The most frequent anaerobic finding in two-month-old infants was Veillonella spp. The Prevotella melaninogenica group also represented early colonizing species, and the frequency increased remarkably during the first year of life, whereas the Prevotella intermedia group organisms seemed to be late colonizers. Fusobacterium nucleatum, non-pigmented Prevotella spp., and Porphyromonas nucleatum were occasional findings in subjects at 2 months but frequent findings in those at one year of age. F. nucleatum was the most frequent strictly anaerobic species in one-year-old infants; other fusobacteria were also occasionally found. The frequency of facultative/micro-aerophilic corroding rods and Capnocytophaga spp. started to increase toward the end of the first year. Except for the common presence of facultative/micro-aerophilic Actinomyces spp., other anaerobic Gram-positive species were only occasionally present in these infants. Once established, early-colonizing species tended to persist in the mouth. Our longitudinal study demonstrated the establishment of several anaerobic species with steadily increasing frequencies during the first year of life.