Powering DNA strand-displacement reactions with a continuous flow reactor
Powering DNA strand-displacement reactions with a continuous flow reactor
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DOI：
10.1007/s11047-020-09795-2
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发表时间：
2020-06-03
影响因子：
2.1
通讯作者：
Schulman, Rebecca
中科院分区：
文献类型：
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作者：
Cui, Xinyu;Scalise, Dominic;Schulman, Rebecca
生命系统需要持续的能量和营养供应才能生存。这些营养物质被摄取，转化为低能量的废物产品，然后排出体外。相比之下，合成的DNA链置换反应通常在初始反应物供应有限的封闭系统中进行。一旦反应物被消耗，所有的净反应停止，系统停止运行。在这里，我们在一个连续流动的反应器中运行DNA链置换反应，注入新的反应物并提取废物，使系统能够动态更新其输出，以响应不断变化的输入。在连续流动反应器内运行DNA链置换反应允许系统重复用于多轮计算，这可能使更复杂的信息处理任务得以执行，包括单轨求反和时序逻辑电路。
Living systems require a sustained supply of energy and nutrients to survive. These nutrients are ingested, transformed into low-energy waste products, and excreted. In contrast, synthetic DNA strand-displacement reactions typically run within closed systems provided with a finite initial supply of reactants. Once the reactants are consumed, all net reactions halt and the system ceases to function. Here we run DNA strand-displacement reactions in a continuous flow reactor, infusing fresh reactants and withdrawing waste, enabling the system to dynamically update its outputs in response to changing inputs. Running DNA strand-displacement reactions inside of continuous flow reactors allows the system to be re-used for multiple rounds of computation, which could enable the execution of more elaborate information processing tasks, including single-rail negation and sequential logic circuits.