An integral membrane protein of the pore membrane domain of the nuclear envelope contains a nucleoporin-like region.
An integral membrane protein of the pore membrane domain of the nuclear envelope contains a nucleoporin-like region.
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DOI：
10.1083/jcb.122.3.513
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发表时间：
1993-08
期刊：
影响因子：
--
通讯作者：
Blobel G
中科院分区：
文献类型：
--
作者：
Hallberg E;Wozniak RW;Blobel G
我们已经确定了一个与WGA结合的大鼠肝核膜的145kD的完整膜蛋白(经SDS-PAGE估计)。我们从纯化的p145中获得了多肽序列，并对多个克隆和一个基因组克隆进行了克隆和测序。根据p145完整的一级结构推算其相对分子质量为120.7 kD。针对p145中代表的合成肽产生的抗体与p145发生单特异性反应。在间接免疫荧光中，这些抗体对核膜进行点状染色。免疫金电镜显示核孔有特殊的纹饰。因此，p145是一种完整的膜蛋白，专门位于核膜的“孔膜”区域。为了指示这一特定位置，并根据其计算的相对分子质量，将该蛋白质命名为POM 121(121kD的孔膜蛋白)。1,199个残基的一级结构显示一个疏水区(残基29-72)，很可能形成一个(或两个相邻的)跨膜片段(S)。大部分蛋白质(残基73-1199)预计不会暴露在池侧，而是暴露在孔膜的孔侧。它包含36个关于不同激酶的共识位点。然而，它最显著的特征是一个重复的五肽基序XFXFG，该基序也被证明存在于几个核孔蛋白中。POM 121的这个核孔素样结构域被认为具有将核孔复合体的成分锚定到孔膜上的功能。
We have identified an integral membrane protein of 145 kD (estimated by SDS-PAGE) of rat liver nuclear envelopes that binds to WGA. We obtained peptide sequence from purified p145 and cloned and sequenced several cDNA clones and one genomic clone. The relative molecular mass of p145 calculated from its complete, cDNA deduced primary structure is 120.7 kD. Antibodies raised against a synthetic peptide represented in p145 reacted monospecifically with p145. In indirect immunofluorescence these antibodies gave punctate staining of the nuclear envelope. Immunogold EM showed specific decoration of the nuclear pores. Thus p145 is an integral membrane protein located specifically in the "pore membrane" domain of the nuclear envelope. To indicate this specific location, and based on its calculated relative molecular mass, the protein is termed POM 121 (pore membrane protein of 121 kD). The 1,199- residue-long primary structure shows a hydrophobic region (residues 29- 72) that is likely to form one (or two adjacent) transmembrane segment(s). The bulk of the protein (residues 73-1199) is predicted to be exposed not on the cisternal side but on the pore side of the pore membrane. It contains 36 consensus sites for various kinases. However, its most striking feature is a repetitive pentapeptide motif XFXFG that has also been shown to occur in several nucleoporins. This nucleoporin- like domain of POM 121 is proposed to function in anchoring components of the nuclear pore complex to the pore membrane.