Low-intensity pulsed ultrasound induced enhanced adipogenesis of adipose-derived stem cells
Low-intensity pulsed ultrasound induced enhanced adipogenesis of adipose-derived stem cells
复制标题
低强度脉冲超声诱导脂肪干细胞的脂肪生成增强。
DOI：
10.1111/cpr.12031
复制
发表时间：
2013-06-01
影响因子：
8.5
通讯作者：
Lin, Y.
中科院分区：
文献类型：
--
作者：
Fu, N.;Yang, X.;Lin, Y.
目的探讨低强度脉冲超声（LIPUS）对脂肪干细胞（ASCs）体外分化的影响。材料与方法将小鼠脂肪细胞诱导成脂后立即或延迟20天用LIPUS处理3天或5天。通过实时荧光定量PCR检测成脂基因PPAR-1和APN的表达。进行免疫荧光（IF）染色以在蛋白质水平上测试PPAR-。结果LIPUS特异性上调了PPAR-1、APN mRNA和蛋白水平。结论在含成脂试剂的培养液中，LIPUS可促进ASC成脂。
Objective The aim of this study was to investigate effects of low-intensity pulsed ultrasound (LIPUS) on differentiation of adipose-derived stem cells (ASCs), in vitro. Materials and methods Murine ASCs were treated with LIPUS for either three or five days, immediately after adipogenic induction, or delayed for 2days. Expression of adipogenic genes PPAR-1, and APN, was examined by real-time PCR. Immunofluorescence (IF) staining was performed to test for PPAR- at the protein level. Results Our data revealed that specific patterns of LIPUS up-regulated levels of both PPAR-1 and APN mRNA, and PPAR- protein. Conclusions In culture medium containing adipogenic reagents, LIPUS enhanced ASC adipogenesis.