Neointima formation after acute vascular injury. Role of counteradhesive extracellular matrix proteins.
Neointima formation after acute vascular injury. Role of counteradhesive extracellular matrix proteins.
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发表时间：
1994
影响因子：
0.9
通讯作者：
M. Majesky
中科院分区：
文献类型：
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作者：
M. Majesky
目前，再狭窄限制了球囊冠状动脉血管成形术的长期有益效果。血管成形术后临床上显着的管腔变窄的两个重要的细胞事件是1)细胞外基质蛋白的产生增加和2)血管平滑肌细胞获得运动表型。在本文中，回顾了急性血管损伤后产生纤维细胞新内膜的平滑肌细胞反应。特别强调与细胞运动和组织修复有关的特殊细胞外基质蛋白。腱蛋白和血小板反应蛋白是大的、模块化的细胞外基质糖蛋白；它们同时具有粘附和反粘附结构域，并且在大鼠颈动脉球囊损伤后平滑肌细胞迁移和新内膜形成过程中高水平表达。生腱蛋白和血小板反应蛋白下调粘着斑（细胞-细胞外基质粘附相互作用点）的组装和活性的能力对于将静止的平滑肌细胞转化为能够在强粘附性血管壁内移动和分裂的细胞可能很重要。此外，生腱蛋白以大的 6 臂寡聚物（六臂体）的形式存在于细胞外基质中，每个六臂体臂中都包含细胞结合域和基质蛋白结合域。生腱蛋白和血小板反应蛋白的大尺寸和多结构域结构表明，这些蛋白质可能特别适合在以下部位形成新生临时基质：1)急性血管损伤后的新内膜形成，2)原发性动脉粥样硬化斑块内的新生长和扩张，以及3)血管成形术后再狭窄中的内膜修复和管腔狭窄。
Restenosis currently limits the long-term beneficial effects of balloon coronary angioplasty. Two important cellular events in the development of clinically significant luminal narrowing after angioplasty are 1) increased production of extracellular matrix proteins and 2) acquisition of a motile phenotype by vascular smooth muscle cells. In this paper, smooth muscle cell responses that produce a fibrocellular neointima after acute vascular injury are reviewed. Particular emphasis is placed on specialized extracellular matrix proteins implicated in cell movement and tissue repair. Tenascin and thrombospondin are large, modular extracellular matrix glycoproteins; they possess both adhesive and counteradhesive domains and are expressed at high levels during smooth muscle cell migration and neointima formation after balloon injury to rat carotid artery. The ability of both tenascin and thrombospondin to down-regulate the assembly and activity of focal adhesions (points of cell-extracellular matrix adhesive interactions) may be important in the conversion of stationary, quiescent smooth muscle cells to cells that are able to move and divide within the strongly adhesive vessel wall. Moreover, tenascin is present in the extracellular matrix as a large 6-armed oligomer (a hexabrachion) that contains both cell-binding and matrix protein-binding domains in each of the hexabrachion arms. The large size and multidomain structure of tenascin and thrombospondin suggest that these proteins may be particularly well suited to form a nascent provisional matrix at sites of 1) neointima formation after acute vascular injury, 2) new growth and expansion within primary atherosclerotic plaques, and 3) intimal repair and luminal narrowing in restenosis after angioplasty.