Chloroplast and nuclear DNA analyses provide insight into the phylogeography and conservation genetics of Camellia nitidissima (Theaceae) in southern Guangxi, China
Chloroplast and nuclear DNA analyses provide insight into the phylogeography and conservation genetics of Camellia nitidissima (Theaceae) in southern Guangxi, China
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叶绿体和核 DNA 分析有助于深入了解中国广西南部金花茶（山茶科）的系统发育地理学和保护遗传学
DOI：
10.1007/s11295-019-1390-1
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发表时间：
2019-12-11
影响因子：
2.4
通讯作者：
Tang, Shaoqing
中科院分区：
文献类型：
--
作者：
Lu, Xuelin;Chen, Hailing;Tang, Shaoqing
山茶是广西南部一种分布有限的高价值地方性观赏植物。这种植物被归类为“易危”，而被称为微卡帕的特殊品种（var.）被归类为“濒危”。本研究通过对分布在广西4个地区的9个种群的一个叶绿体片段和2个单拷贝核DNA序列的系统地理分析，研究了黑桫椤的遗传结构。我们进行这项研究是为了促进制定适当的保护策略来保护这种植物。核DNA分析表明，微笛鲷与黑笛鲷的遗传变异明显不同。叶绿体和核标记在三个不相连的地理区域显示出高度的遗传变异，表明该品种目前的遗传结构反映了长期的分离和有限的基因在所调查区域间的流动。我们建议保护计划承认微笛卡帕，并将三个不相交的地理种群群作为三个独立的管理单位来对待。
Camellia nitidissima is a high-value endemic ornamental plant with a limited distribution in the south of Guangxi, China. This plant is classified as 'vulnerable', and the particular variety (var.) known as microcarpa is classified as 'endangered'. In this study, we used phylogeographic analysis of one chloroplast fragment and two single-copy nuclear DNA sequences to study the genetic structure of C. nitidissima samples from nine populations distributed across four regions in Guangxi. We carried out this study to facilitate the development of appropriate conservation strategies for the protection of this plant. Analysis of nuclear DNA showed that the genetic variation in var. microcarpa was distinct from that in var. nitidissima. Chloroplast and nuclear markers showed high levels of genetic variation among three disjunct geographical regions of var. nitidissima, indicating that the current genetic structure of this variety reflects long-term isolation and limited gene flow across the regions investigated. We recommend that conservation programmes recognise var. microcarpa and that the three disjunct geographical population groups of var. nitidissima be treated as three separate management units.