Antiviral antibody profiling by high-density protein arrays.
Antiviral antibody profiling by high-density protein arrays.
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DOI：
10.1002/pmic.201400612
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发表时间：
2015-06
期刊：
影响因子：
3.4
通讯作者：
LaBaer J
中科院分区：
文献类型：
--
作者：
Bian X;Wiktor P;Kahn P;Brunner A;Khela A;Karthikeyan K;Barker K;Yu X;Magee M;Wasserfall CH;Gibson D;Rooney ME;Qiu J;LaBaer J
病毒感染会引起抗病毒抗体，并与各种慢性病有关。检测这些抗体有助于诊断、治疗感染和了解病毒相关疾病的发病机制。在这项工作中，我们使用了一种新型的高密度核酸可编程蛋白阵列(HD-NAPPA)平台来检测抗病毒抗体。在一系列微观反应室中，从编码蛋白质的质粒直接原位表达单个病毒蛋白。蛋白质展示和血清反应的质量通过比较打印批次内和阵列间的相关性来确保，平均相关系数分别为0.91和0.96。HD-NAPPA在平板玻片和酶联免疫吸附试验中的信号背景比(S/B)高于标准NAPPA。本文分析了幼年特发性关节炎(JIA)和1型糖尿病(T1D)患者对来自25种不同病毒的761种抗原的抗体反应。在患者和健康对照组之间检测到了共同的和独特的抗体反应模式。我们相信，HD-病毒-NAPPA将以前所未有的规模研究宿主和病原体的相互作用，并阐明病原体感染在疾病发展中的作用。
Viral infections elicit anti-viral antibodies and have been associated with various chronic diseases. Detection of these antibodies can facilitate diagnosis, treatment of infection and understanding of the mechanisms of virus associated diseases. In this work, we assayed anti-viral antibodies using a novel high density-nucleic acid programmable protein array (HD-NAPPA) platform. Individual viral proteins were expressed in situ directly from plasmids encoding proteins in an array of microscopic reaction chambers. Quality of protein display and serum response was assured by comparing intra- and inter- array correlation within or between printing batches with average correlation coefficients of 0.91 and 0.96, respectively. HD-NAPPA showed higher signal to background (S/B) ratio compared with standard NAPPA on planar glass slides and ELISA. Antibody responses to 761 antigens from 25 different viruses were profiled among patients with juvenile idiopathic arthritis (JIA) and type 1 diabetes (T1D). Common as well as unique antibody reactivity patterns were detected between patients and healthy controls. We believe HD-viral-NAPPA will enable the study of host-pathogen interactions at unprecedented dimensions and elucidate the role of pathogen infections in disease development.