Cellular niches controlling B lymphocyte behavior within bone marrow during development
Cellular niches controlling B lymphocyte behavior within bone marrow during development
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DOI：
10.1016/j.immuni.2004.05.001
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发表时间：
2004-06-01
期刊：
影响因子：
32.4
通讯作者：
Nagasawa, T
中科院分区：
文献类型：
--
作者：
Tokoyoda, K;Egawa, T;Nagasawa, T
在骨髓中，造血被认为依赖于特殊的微环境，即维持血细胞的生态位。然而，壁龛的身份和血细胞与壁龛的相互作用仍然知之甚少。在这里，我们确定了B淋巴生成的阶段特异性细胞壁龛。最早的前体，前-前B细胞和终末期B细胞，浆细胞需要CXC趋化因子配体（CXCL）12。表达cxcl12的细胞是一小群基质细胞，分布在骨髓中，与表达白细胞介素(IL)-7的细胞有一定距离。多能造血祖细胞附着在表达cxcl12的细胞突起上，前-前- b细胞附着在其细胞体上。需要IL-7的成熟前b细胞已经离开并靠近表达IL-7的细胞。浆细胞再次播种表达cxcl12的细胞。我们证明了B淋巴细胞在发育过程中在骨髓中特定生态位之间的特征定位和运动，并表明CXCL12维持了生态位中的细胞。
In bone marrow, hematopoiesis is thought to depend on special microenvironments known as niches that maintain blood cells. However, the identity of niches and interaction of blood cells with niches remain poorly understood. Here we identify stage-specific cellular niches for B lymphopoiesis. The earliest precursors, pre-pro-B cells and end-stage B cells, plasma cells require CXC chemokine ligand (CXCL)12. CXCL12-expressing cells are a small population of stromal cells, scattered throughout bone marrow and located some distance from the cells expressing interleukin (IL)-7. Multipotent hematopoietic progenitors are attached to the processes of CXCL12-expressing cells and pre-pro-B cells adjoin their cell bodies. Maturer pro-B cells that require IL-7 have moved away and adjoin the IL-7-expressing cells. Plasma cells again seed CXCL12-expressing cells. We demonstrate the B lymphocyte characteristic location and movement between specific niches within bone marrow during development and suggest that CXCL12 maintains the cells in the niche.