Gut microbiota metabolic potential correlates with body size between mulberry-feeding lepidopteran pest species
Gut microbiota metabolic potential correlates with body size between mulberry-feeding lepidopteran pest species
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肠道微生物群代谢潜力与食桑鳞翅目害虫物种的体型相关
DOI：
10.1002/ps.5642
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发表时间：
2019-11-25
影响因子：
4.1
通讯作者：
Shao, Yongqi
中科院分区：
文献类型：
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作者：
Chen, Bosheng;Xie, Sen;Shao, Yongqi
背景许多害虫依靠微生物共生体来获取营养或防御，从而使它们能够开发新的食物来源并降解环境中的外来物质，包括农药。鳞翅目是昆虫种类最多的类群之一，也是重要的农业害虫之一，但对鳞翅目微生物，特别是功能性状的研究还很少。在这里，我们提供了多个桑树食性鳞翅目昆虫物种肠道微生物区系的综合特征，解析了群落结构和代谢潜力。结果鳞翅目幼虫肠道内细菌含量丰富。然而，尽管它们的饲料相同，但四种主要桑树害虫的细菌群落结构不同，这表明寄主对微生物群落的影响是特定的。群落水平的代谢重建进一步表明，尽管分类组成差异很大，但碳水化合物和氨基酸代谢和膜转运蛋白是所有样品中肠道细菌的关键功能，可能在幼虫肠道中发挥基础作用。此外，肠道细菌预测基因本体论的主坐标分析(PCoA)揭示了与这些桑树害虫相关的微生物群的特殊特征，将其分为两个不同的类群(大鳞翅目和小鳞翅目)。当进一步涉及鳞翅目物种时，这种模式变得更加突出。结论肠道微生物区系代谢功能与幼虫大小密切相关，大种肠道微生物区系代谢主要集中在萜类和多酮类的代谢、外源物质的生物降解和代谢，而小类幼虫的核苷酸代谢明显增强。我们的报告为揭示这一臭名昭著的害虫群体中寄主表型和微生物共生之间的关系铺平了道路。(三)2019年化学工业学会
BACKGROUND Many insect pests rely on microbial symbionts to obtain nutrients or for defence, thereby allowing them to exploit novel food sources and degrade environmental xenobiotics, including pesticides. Although Lepidoptera is one of the most diverse insect taxa and includes important agricultural pests, lepidopteran microbiotas, particularly functional traits, have not been studied widely. Here, we provide a comprehensive characterization of the gut microbiota across multiple mulberry-feeding lepidopteran species, resolving both community structure and metabolic potential. RESULTS Our results indicate abundant bacteria inside the gut of larval Lepidoptera. However, even though they were fed the same diet, the structures of the bacterial communities differed in four major mulberry pest species, suggesting host-specific effects on microbial associations. Community-level metabolic reconstructions further showed that although taxonomic composition varied greatly, carbohydrate and amino acid metabolism and membrane transporter were key functional capabilities of the gut bacteria in all samples, which may play basic roles in the larval gut. In addition, principal coordinate analysis (PCoA) of gut bacterial-predicted gene ontologies revealed specialized features of the microbiota associated with these mulberry pests, which were divided into two distinct clusters (macrolepidopterans and microlepidopterans). This pattern became even more prominent when further Lepidoptera species were involved. CONCLUSIONS A suite of gut microbiota metabolic functions significantly correlated with larval size; the metabolism of terpenoids and polyketides, xenobiotics biodegradation and metabolism were specifically enriched in large species, while small larvae had enhanced nucleotide metabolism. Our report paves the way for uncovering the correlation between host phenotype and microbial symbiosis in this notorious insect pest group. (c) 2019 Society of Chemical Industry