AN IMMUNOCYTOCHEMICAL STUDY OF CAT RETINAL MULLER CELLS IN CULTURE
AN IMMUNOCYTOCHEMICAL STUDY OF CAT RETINAL MULLER CELLS IN CULTURE
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DOI：
10.1016/0014-4835(88)90068-1
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发表时间：
1988-12-01
影响因子：
3.4
通讯作者：
FISHER, SK
中科院分区：
文献类型：
--
作者：
LEWIS, GP;KASKA, DD;FISHER, SK
Muller细胞是在脊椎动物视网膜中发现的特化放射状胶质细胞，从成年猫视网膜中酶促分离并在培养物中生长。在培养4小时至3个月的时间范围内处理细胞用于免疫荧光显微镜检查。在培养的细胞中检测到对胶质细胞酸性蛋白、细胞视黄醇结合蛋白、谷氨酰胺合成酶、碳酸酐酶C和α晶体蛋白（已知在Muller细胞中发现的所有蛋白）具有特异性的抗体的标记。培养细胞的免疫印迹分析显示对应于抗原的适当分子量的单一蛋白条带。
Muller cells, the specialized radial glia found in vertebrate retinas, were enzymatically dissociated from adult cat retinas and grown in culture. The cells processed for immunofluorescence microscopy at times ranging from 4 hr to 3 months in culture. Labeling with antibodies specific to glial fibrillary acidic protein, cellular retinaldehyde binding protein, glutamine synthetase, carbonic anhydrase C and alpha crystallin, all proteins known to be found in Muller cells, was detected in the cultured cells. Immunoblot analysis of the cultured cells showed single protein bands corresponding to the appropriate molecular weights of the antigens.