Differential placental gene expression in preeclampsia
Differential placental gene expression in preeclampsia
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DOI：
10.1016/j.ajog.2008.04.020
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发表时间：
2008-11-01
影响因子：
9.8
通讯作者：
Williams, Michelle A.
中科院分区：
文献类型：
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作者：
Enquobahrie, Daniel A.;Meller, Margaret;Williams, Michelle A.
目的：与先兆子痫相关的候选基因尚未得到充分描述。我们进行了微阵列和验证性定量实时聚合酶链反应研究，以调查先兆子痫的整体胎盘基因表达。研究设计：从 18 名先兆子痫病例和 18 名血压正常对照受试者收集的胎盘样本中提取 RNA。使用代表 22,000 个基因的寡核苷酸探针来测量每个样本中的基因表达。通过学生 t 检验、倍数变化评估和微阵列显着性分析来评估差异基因表达。评估差异表达基因的功能和功能关系。结果：参与免疫系统、炎症、氧化应激、信号传导、生长和发育途径的基因（n = 58）在先兆子痫中差异表达。这些基因包括先前描述的候选基因（如瘦素）、具有相关功能的潜在候选基因（如CYP11A）和新基因（如CDKN1C）。结论：具有不同功能的基因（候选基因和新基因）的表达与先兆子痫风险相关，反映了复杂的发病机制。
OBJECTIVE: Candidate genes that are associated with preeclampsia have not been described fully. We conducted microarray and confirmatory quantitative real time polymerase chain reaction studies to investigate global placental gene expression in preeclampsia.STUDY DESIGN: RNA was extracted from placental samples that were collected from 18 preeclampsia cases and 18 normotensive control subjects. Oligonucleotide probes that represented 22,000 genes were used to measure gene expression in each sample. Differential gene expression was evaluated with the Student t test, fold change assessment, and significance analysis of microarrays. Functions and functional relationships of differentially expressed genes were evaluated.RESULTS: Genes (n = 58) that participated in immune system, inflammation, oxidative stress, signaling, growth, and development pathways were expressed differentially in preeclampsia. These genes included previously described candidate genes (such as leptin), potential candidate genes with related functions (such as CYP11A) and novel genes (such as CDKN1C).CONCLUSION: Expression of genes (both candidate and novel) with diverse functions is associated with preeclampsia risk, which reflects the complex pathogenesis.