Selenium-Enriched Probiotics Improves Murine Male Fertility Compromised by High Fat Diet
Selenium-Enriched Probiotics Improves Murine Male Fertility Compromised by High Fat Diet
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富硒益生菌可改善因高脂肪饮食而受损的小鼠雄性生育能力
DOI：
10.1007/s12011-011-9308-2
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发表时间：
2012-06-01
影响因子：
3.9
通讯作者：
Haung, Kehe
中科院分区：
文献类型：
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作者：
Ibrahim, Hala A. M.;Zhu, Yongxing;Haung, Kehe
总共 75 只雄性小鼠被分为 5 组，每组 15 只，采用完全随机的实验设计，研究益生菌、无机硒和富硒益生菌对高脂血症状态下雄性生育力的影响。第1组的小鼠喂食正常的基础饮食并作为阴性对照。第2组的小鼠被喂食高脂肪饮食并作为阳性对照。第3、4和5组的小鼠分别喂食添加益生菌、无机硒和富硒益生菌的高脂饮食。高脂肪饮食由15%猪油、1%胆固醇、0.3%胆酸和83.7%基础饮食组成。富硒益生菌中90%以上的硒以有机硒的形式存在。小鼠喂食这些饮食 75 天后，测量了血清总胆固醇、甘油三酯、低密度脂蛋白、高密度脂蛋白和睾酮水平，以及精子指数（计数、活力和异常）、阴茎长度、睾丸重量和组织病理学。结果显示，高脂饮食喂养的小鼠血清总胆固醇、甘油三酯和低密度脂蛋白显着升高（P<0.01），而高密度脂蛋白显着降低。高脂肪饮食导致血清睾酮水平下降，精液质量下降，生精小管萎缩变性。没有观察到对阴茎长度或睾丸相对重量的影响。在高脂饮食中补充益生菌、无机硒或富硒益生菌可通过减轻睾丸组织损伤、提高血清睾酮水平、改善精子指数等方式显着减轻（P< 0.05）高脂血症的不良影响。结论：高脂血症对男性生育能力有显着的不利影响，通过添加益生菌、无机硒或富硒益生菌的饮食可以不同程度地改善这种影响。富硒益生菌或无机硒补充剂比益生菌补充剂效果更好，可用于改善因高脂血症或肥胖而受损的动物和人类男性生育能力。
A total of 75 male mice were allotted to five groups of 15 each in a completely randomized experimental design to study the effects of probiotics, inorganic selenium, and selenium-enriched probiotics on male fertility in hyperlipidemic status. The mice in group 1 were fed a normal basal diet and served as negative control. The mice in group 2 were fed a high fat diet and served as positive control. The mice in groups 3, 4, and 5 were fed the high fat diet supplemented with probiotics, inorganic selenium, and selenium-enriched probiotics, respectively. The high fat diet was composed of 15% lard, 1% cholesterol, 0.3% cholic acid, and 83.7% basal diet. Over 90% of the selenium in the selenium-enriched probiotics was present in forms of organic selenium. After the mice were fed these diets for 75 days, serumal total cholesterol, triglycerides, low density lipoprotein, high density lipoprotein, and testosterone levels, plus sperm index (count, motility and abnormalities), penis length, and weight and histopathology of testes were measured. The results showed that in the mice fed the high fat diet were significant (P< 0.01) elevations of serumal total cholesterol, triglycerides and low density lipoprotein, and decreases of high density lipoprotein. The high fat diet caused a decline in serumal testosterone level, reduced semen quality, and atrophy and degeneration of seminiferous tubules. No effects on penis length or relative weight of testis were observed. Supplementation of probiotics, inorganic selenium, or selenium-enriched probiotics to the high fat diet significantly alleviated (P< 0.05) the adverse effects of hyperlipidemia by reducing testicular tissue injury, increasing serumal testosterone level, and improving sperm indexes. It was concluded that hyperlipidemia had significant adverse effects on male fertility, which could be ameliorated at various degrees by feeding the diets supplemented with probiotics, inorganic selenium, or selenium-enriched probiotics. Selenium-enriched probiotics or inorganic selenium supplementation gave better results than probiotics supplementation and may be used to improve animal and human male fertility compromised by hyperlipidemia or obesity.