Healthy human T-Cell Responses to Aspergillus fumigatus antigens.
Healthy human T-Cell Responses to Aspergillus fumigatus antigens.
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DOI：
10.1371/journal.pone.0009036
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发表时间：
2010-02-17
期刊：
影响因子：
3.7
通讯作者：
Marr KA
中科院分区：
文献类型：
--
作者：
Chaudhary N;Staab JF;Marr KA
Aspergillus fumigatus is associated with both invasive and allergic pulmonary diseases, in different hosts. The organism is inhaled as a spore, which, if not cleared from the airway, germinates into hyphal morphotypes that are responsible for tissue invasion and resultant inflammation. Hyphae secrete multiple products that function as antigens, evoking both a protective (TH1–TH17) and destructive allergic (TH2) immunity. How Aspergillus allergens (Asp f proteins) participate in the development of allergic sensitization is unknown. To determine whether Asp f proteins are strictly associated with TH2 responses, or represent soluble hyphal products recognized by healthy hosts, human T cell responses to crude and recombinant products were characterized by ELISPOT. While responses (number of spots producing IFN-γ, IL-4 or IL-17) to crude hyphal antigen preparations were weak, responses to recombinant Asp f proteins were higher. Recombinant allergens stimulated cells to produce IFN-γ more so than IL-4 or IL-17. Volunteers exhibited a diverse CD4+ and CD8+ T cell antigen recognition profile, with prominent CD4 TH1-responses to Asp f3 (a putative peroxismal membrane protein), Asp f9/16 (cell wall glucanase), Asp f11 (cyclophilin type peptidyl-prolyl isomerase) and Asp f22 (enolase). Strong IFN-γ responses were reproduced in most subjects tested over 6 month intervals. Products secreted after conidial germination into hyphae are differentially recognized by protective T cells in healthy, non-atopic individuals. Defining the specificity of the human T cell repertoire, and identifying factors that govern early responses may allow for development of novel diagnostics and therapeutics for both invasive and allergic Aspergillus diseases.
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DOI：
10.1056/nejmoa0802629
发表时间：
2008-10-23
期刊：
The New England journal of medicine
影响因子：
--
作者：
Bochud PY;Chien JW;Marr KA;Leisenring WM;Upton A;Janer M;Rodrigues SD;Li S;Hansen JA;Zhao LP;Aderem A;Boeckh M
通讯作者：
Boeckh M
影响因子：
12.4
作者：
Knutsen, AP;Hutcheson, PS;Kurup, VP
通讯作者：
Kurup, VP
影响因子：
2.9
作者：
Montagnoli, C;Sandini, S;La Valle, R
通讯作者：
La Valle, R
影响因子：
30.5
作者：
LeibundGut-Landmann, Salome;Gross, Olaf;Sousa, Caetano Reis e
通讯作者：
Sousa, Caetano Reis e
影响因子：
20.3
作者：
LeibundGut-Landmann, Salome;Osorio, Fabiola;Sousa, Caetano Reis e
通讯作者：
Sousa, Caetano Reis e