Light absorption by anthocyanins in juvenile, stressed, and senescing leaves.
Light absorption by anthocyanins in juvenile, stressed, and senescing leaves.
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DOI：
10.1093/jxb/ern230
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发表时间：
2008
影响因子：
6.9
通讯作者：
Naqvi KR
中科院分区：
文献类型：
--
作者：
Merzlyak MN;Chivkunova OB;Solovchenko AE;Naqvi KR
研究了挪威枫树(Acer Platanoides L.)、椰子(Cotoneaster Alaunica GoLite)、金缕梅(Corylus avellana L.)、山茱萸(Cornus alba L.)和爬山虎(Parthenocisss ququiefolia(L.)Plch.)，在不同的色素组成和个体发育的不同阶段进行了研究。用完整花青素和去氰化叶的分光光度法和叶横切面液泡显微分光光度法估算的活体花色苷吸收峰在537~542 nm之间，与酸性水-甲醇提取物的相应峰相比，红移了5~20 nm。在非衰老叶片中，花色苷在500-600 nm之间有较强的吸收(表观带宽为70-80 nm)。大体上，花色苷在叶片中的吸收遵循兰伯特-比尔定律的修正形式，呈现线性趋势，直到近50nmolcm−2的含量，从而允许对花色苷含量的非侵入性测定。花色苷在550 nm处的表观比吸收系数与物种无关。枫叶在秋季衰老过程中，花色苷对550 nm总吸光量的贡献。讨论了液泡型花色苷的光保护作用，特别考虑了它们在叶片中的分布；还评价了主要定位于白杨和金线莲叶片表皮细胞中的花青素对辐射的屏蔽作用。
The optical properties of leaves from five species, Norway maple (Acer platanoides L.), cotoneaster (Cotoneaster alaunica Golite), hazel (Corylus avellana L.), Siberian dogwood (Cornus alba L.), and Virginia creeper (Parthenocissus quinquefolia (L.) Planch.), differing in pigment composition and at different stages of ontogenesis, were studied. Anthocyanin absorption maxima in vivo, as estimated with spectrophotometry of intact anthocyanic versus acyanic leaves and microspectrophotometry of vacuoles in the leaf cross-sections, were found between 537 nm and 542 nm, showing a red shift of 5–20 nm compared with the corresponding maxima in acidic water–methanol extracts. In non-senescent leaves, strong anthocyanin absorption was found between 500 nm and 600 nm (with a 70–80 nm apparent bandwidth). By and large, absorption by anthocyanin in leaves followed a modified form of the Lambert–Beer law, showing a linear trend up to a content of nearly 50 nmol cm−2, and permitting thereby a non-invasive determination of anthocyanin content. The apparent specific absorption coefficients of anthocyanins at 550 nm showed no substantial dependence on the species. Anthocyanin contribution to total light absorption at 550 nm was followed in maple leaves in the course of autumn senescence. Photoprotection by vacuolar anthocyanins is discussed with special regard to their distribution within a leaf; radiation screening by anthocyanins predominantly localized in the epidermal cells in A. platanoides and C. avellana leaves was also evaluated.
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影响因子：
6.4
作者：
Gould, KS;Vogelmann, TC;Clearwater, MJ
通讯作者：
Clearwater, MJ
影响因子：
1.4
作者：
Solovchenko, AE;Chivkunova, OB;Reshetnikova, IV
通讯作者：
Reshetnikova, IV
影响因子：
7.3
作者：
Nishio, JN
通讯作者：
Nishio, JN
影响因子：
3.1
作者：
Merzlyak, M;Solovchenko, A;Pogosyan, S
通讯作者：
Pogosyan, S
DOI：
10.1016/j.jphotobiol.2004.03.003
发表时间：
2004-05-27
影响因子：
5.4
作者：
Merzlyak, MN;Melo, TB;Naqvi, KR
通讯作者：
Naqvi, KR