Multielemental speciation analysis of fungi porcini (Boletus edulis) mushroom by size exclusion liquid chromatography with sequential on-line UV-ICP-MS detection.
Multielemental speciation analysis of fungi porcini (Boletus edulis) mushroom by size exclusion liquid chromatography with sequential on-line UV-ICP-MS detection.
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DOI：
10.1021/jf035118o
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发表时间：
2004-02
影响因子：
6.1
通讯作者：
R. Wuilloud;Sasi S. Kannamkumarath;J. Caruso
中科院分区：
文献类型：
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作者：
R. Wuilloud;Sasi S. Kannamkumarath;J. Caruso
一种分析方法，以确定分子量（MW）分布模式的几种元素之间的不同化合物存在于常用食用蘑菇在这项工作中。使用基于尺寸排阻液相色谱（SEC）在线耦合UV和电感耦合等离子体质谱（ICP-MS）检测的联用技术。通过UV和ICP-MS连续检测确认了元素与高和低MW级分的关联。通过将100微升样品体积注入Superdex 75柱进行级分的分离。评价了不同移动的相对分离的影响。此外，三种不同的提取条件，包括0.05 mol L（-1）NaOH，0.05 mol L（-1）HCl，和热水在60摄氏度的蘑菇样品中提取的元素物种。根据所用浸提条件，在色谱图中观察到显著差异。对SEC-UV-ICP-MS联用中涉及的实验变量进行了优化。应用该方法研究了美味牛肝菌中Bi、Co、Cu、Fe、I、Mo、Ni、Se、Zn等元素的形态分布规律。在这项工作中获得的结果表明，一个重要的协会的大部分元素的高分子量馏分。
An analytical methodology to determine the molecular weight (MW) distribution patterns of several elements among different compounds present in commonly consumed edible mushrooms is presented in this work. A hyphenated technique based on size exclusion liquid chromatography (SEC) coupled on-line to UV and inductively coupled plasma mass spectrometry (ICP-MS) detection was used. The association of the elements to high and low MW fractions was confirmed with sequential detection by UV and ICP-MS. Separation of the fractions was performed by injecting a 100 microL sample volume to a Superdex 75 column. The effect of different mobile phases on the separation was evaluated. Additionally, three different extraction conditions including 0.05 mol L(-1) NaOH, 0.05 mol L(-1) HCl, and hot water at 60 degrees C were applied to extract the elemental species from the mushroom samples. Significant differences were observed in the chromatograms depending on the extraction conditions utilized. Optimization of the experimental variables involved in the SEC-UV-ICP-MS coupling was carried out. The method was applied to investigate the fractionation patterns of Bi, Co, Cu, Fe, I, Mo, Ni, Se, and Zn in fungi porcini (Boletus edulis) mushroom. The results obtained in this work indicate an important association of most of the elements to high MW fractions.