EFFECTS OF QUINOLONES ON NUCLEOID SEGREGATION IN ESCHERICHIA-COLI
EFFECTS OF QUINOLONES ON NUCLEOID SEGREGATION IN ESCHERICHIA-COLI
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DOI：
10.1128/aac.35.12.2645
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发表时间：
1991-12-01
影响因子：
4.9
通讯作者：
BERTASSO, A
中科院分区：
文献类型：
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作者：
GEORGOPAPADAKOU, NH;BERTASSO, A
以氟罗沙星（Ro 23-6240，AM 833）为原型化合物，考察了喹诺酮类抗生素对生长中大肠杆菌类核分离的影响。 在接近其MIC并诱导生长停滞和增殖的水平下，氟罗沙星导致大的类核出现在中细胞中，表明抑制类核分离。 随着氟罗沙星浓度的增加，类核逐渐变小，表明DNA复制的抑制。 去除氟罗沙星恢复正常的细胞和核形态的细丝与大的类核，但不是在细丝与小的类核。 结果与在低喹诺酮浓度下抑制染色体脱连环（抑菌作用）和在高浓度下抑制DNA超螺旋（杀菌作用）一致。
The effects of quinolone antibiotics on nucleoid segregation in growing Escherichia coli were examined by using fleroxacin (Ro 23-6240, AM 833) as a prototype compound. At levels that were close to its MIC and induced growth arrest and filamentation, fleroxacin caused large nucleoids to appear in midcell, suggesting inhibition of nucleoid segregation. With increasing fleroxacin concentrations, nucleoids became progressively smaller, suggesting inhibition of DNA replication. Removal of fleroxacin restored normal cell and nucleoid morphology in filaments with large nucleoids but not in filaments with small nucleoids. The results are consistent with inhibition of chromosome decatenation at low quinolone concentrations (bacteriostatic effect) and DNA supercoiling at high concentrations (bactericidal effect).