Draconibacterium orientale gen. nov., sp nov., isolated from two distinct marine environments, and proposal of Draconibacteriaceae fam. nov.
Draconibacterium orientale gen. nov., sp nov., isolated from two distinct marine environments, and proposal of Draconibacteriaceae fam. nov.
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东方龙杆菌属
DOI：
10.1099/ijs.0.056812-0
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发表时间：
2014-05-01
影响因子：
2.8
通讯作者：
Chen, Guan-Jun
中科院分区：
文献类型：
--
作者：
Du, Zong-Jun;Wang, Ying;Chen, Guan-Jun
从两种不同的海洋环境中分离到的两种细菌菌株FH 5（T）和SS 4的分类特征进行了测定。这些细菌是革兰氏染色阴性的兼性厌氧杆菌。生长发生在20-40 ℃（最适，28-32 ℃），pH 5.5-9.0（最适，pH 7.0-7.5）和1- 7%NaCl（最适，2-4%）存在下。细胞脂肪酸主要为anteiso-C-15：0和iso-C-（15：0）。甲基萘醌7（MK-7）是唯一的呼吸醌。主要极性脂质为磷脂酰乙醇胺、一种未知磷脂和一种未知脂质。菌株FH 5（T）和SS 4的DNA G+C含量均为42.0mol%。DNA-DNA杂交研究结果表明，FH 5（T）和SS 4基因组具有大于95%的相关性。这些菌株在拟杆菌纲内形成了一个独特的系统系，与其最近的具有有效公布名称的亲属的序列相似性低于89.4%。根据生理生化特征、16 S rRNA基因序列和化学性质，将东方龙杆菌（Draconibacterium orientale gen. nov.）新种，拟杆菌纲中的菌株FH 5（T）（=DSM 25947（T）=CICC 10585（T））作为模式菌株。此外，还发现了一个新的科，即龙杆菌科Draconibacteriaceae fam.十一月，建议容纳Draconibacterium gen. nov.
The taxonomic characteristics of two bacterial strains, FH5(T) and SS4, isolated from enrichment cultures obtained from two distinct marine environments, were determined. These bacteria were Gram-stain-negative, facultatively anaerobic rods. Growth occurred at 20-40 degrees C (optimum, 28-32 degrees C), pH 5.5-9.0 (optimum, pH 7.0-7.5) and in the presence of 1-7% NaCl (optimum, 2-4%). The major cellular fatty acids were anteiso-C-15:0 and iso-C-(15:0). Menaquinone 7 (MK-7) was the sole respiratory quinone. The major polar lipids were phosphatidylethanolamine, an unkown phospholipid and an unknown lipid. The DNA G+C contents of strains FH5(T) and SS4 were both determined to be 42.0 mol%. The results of DNA DNA hybridization studies indicated that the FH5(T) and SS4 genomes share greater than 95% relatedness. The strains formed a distinct phyletic line within the class Bacteroidia, with less than 89.4% sequence similarity to their closest relatives with validly published names. On the basis of physiological and biochemical characteristics, 16S rRNA gene sequences and chemical properties, a novel genus and species, Draconibacterium orientale gen. nov., sp. nov., within the class Bacteroidia, are proposed, with strain FH5(T) (=DSM 25947(T)=CICC 10585(T)) as the type strain. In addition, a new family, Draconibacteriaceae fam. nov., is proposed to accommodate Draconibacterium gen. nov.