Application of the statistical test of equivalent pathways (STEP) method to the triple quadrupole mass spectrometer.
Application of the statistical test of equivalent pathways (STEP) method to the triple quadrupole mass spectrometer.
复制标题
等效路径统计检验（STEP）方法在三重四极杆质谱仪中的应用。
DOI：
10.1002/rcm.3229
复制
发表时间：
2007
期刊：
影响因子：
--
通讯作者：
Desaire,Heather
中科院分区：
文献类型：
--
作者：
Dalpathado,DilushaS;Chang,Qing;Burkett,ColleenM;Bandu,MaryL;Desaire,Heather
最近，我们展示了一种新的方法，STEP（等效途径统计检验）分析，它区分了在四极离子阱质谱仪上的串联质谱（MS/MS）实验中获得的第一代产品离子（一次产品离子）和第二代产品离子（二次产品离子）。这里提出的研究定义了如何使STEP方法适应更常用的质量分析仪，三重四极杆。开发了新的离子活化条件，使STEP方法适用于多肽和碳水化合物的三重四极杆质谱仪。这种方法的应用是有用的，因为它提供了一个有效的方法来区分初级和次级离子的仪器。在接受STEP分析的离子总数中，该方法正确识别出96%的离子为一级或二级，表明该分析对碳水化合物和肽在三重四极杆质谱仪上进行碰撞诱导解离（CID）是有效的。版权所有©2007 John Wiley & Sons， Ltd
Recently, we demonstrated a new method, STEP (Statistical Test of Equivalent Pathways) analysis, which differentiates first‐generation product ions (primary product ions) from second‐generation product ions (secondary product ions) obtained in tandem mass spectrometric (MS/MS) experiments on a quadrupole ion trap mass spectrometer. The study presented here defines how to adapt the STEP method to a more routinely used mass analyzer, the triple quadrupole. New ion activation conditions were developed to adapt the STEP method to the triple quadrupole mass spectrometer using peptides and carbohydrates. The application of this method to the triple quadrupole is useful because it provides an efficient approach to differentiate primary and secondary ions on this instrument. Out of the total number of ions that were subjected to the STEP analysis, this method correctly identified 96% of ions as primary or secondary, indicating that this analysis is effective for carbohydrates and peptides undergoing collision‐induced dissociation (CID) on a triple quadrupole mass spectrometer. Copyright © 2007 John Wiley & Sons, Ltd.