Angiotensin II receptors in the human adrenal gland
Angiotensin II receptors in the human adrenal gland
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DOI：
10.1080/07435809609043718
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发表时间：
1996-01-01
影响因子：
2.1
通讯作者：
GalloPayet, N
中科院分区：
文献类型：
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作者：
Breault, L;Lehoux, JG;GalloPayet, N
在本研究中，我们研究了血管紧张素II （Ang II）受体在摘除后立即冷冻的人肾上腺中的分布和药理特性。放射自显影研究表明，Ang II受体存在于整个腺体。与AT(1)受体特异性拮抗剂DUP 753和AT(2)受体特异性拮抗剂PD 123319共孵育，发现Ang II受体主要为2型。妊娠16周后，在腺体周围检测到AT(1)受体。竞争研究和Scatchard分析显示，高亲和力AT(2)结合位点的同质群体（Kd = 0.68 +/- 0.1 nM）。结合能力从14周时的1080 +/- 304 fmol/mg蛋白下降到21周时的275 +/- 55 fmol/mg蛋白。这些结果与成人腺体的结果不同，成人腺体的放射自显影研究显示AT(1)受体主要存在于肾小球带，AT(2)受体主要存在于髓质。这些数据提示，AT(2)受体可能参与了人胎儿肾上腺的形态或功能分化。
In the present study, we have characterized distribution and pharmacological properties of angiotensin II (Ang II) receptors in human adrenals frozen immediately after removal. Autoradiographic studies indicate that Ang II receptors are present throughout the gland. Co-incubations with DUP 753, a specific antagonist of the AT(1) receptor, and with PD 123319, a specific antagonist of the AT(2) receptor, reveal that Ang II receptors are mainly of type 2. The AT(1) receptors are detected after 16 weeks of gestation at the periphery of the gland. Competition studies and Scatchard analysis reveal a homogenous population of high affinity AT(2) binding sites (Kd = 0.68 +/- 0.1 nM). Binding capacities decrease from 1080 +/- 304 fmol/mg protein at 14 weeks to 275 +/- 55 fmol/mg protein at 21 weeks. These results differ from those obtained in adult glands where autoradiographic studies reveal that the AT(1) receptors are found mainly in the zona glomerulosa and AT(2) receptors mainly in the medulla. These data suggest that the AT(2) receptors could be involved in the morphological or functional differentiation of the human fetal adrenal gland.