Isolation of Endogenous Peptides from Cultured Cell Conditioned Media for Mass Spectrometry
Isolation of Endogenous Peptides from Cultured Cell Conditioned Media for Mass Spectrometry
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从培养细胞条件培养基中分离内源肽用于质谱分析
DOI：
10.1007/978-1-4939-7537-2_3
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发表时间：
2018
影响因子：
--
通讯作者：
Osaki Tsukasa
中科院分区：
文献类型：
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作者：
Sasaki Kazuki;Tsuchiya Takashi;Osaki Tsukasa
由培养的细胞调节的培养基代表了富含内源性肽的极好来源。组成肽的无偏质谱分析提供了一个机会，看看蛋白水解事件，如生物活性肽加工，膜蛋白胞外域脱落，甚至调节膜内蛋白水解。如果大规模进行，肽组学具有精确定位初级切割位点的潜力。本文描述了在质谱分析之前从培养的细胞条件培养基中分离肽的方法。
Media conditioned by cultured cells represent an excellent source rich in endogenous peptides. Unbiased mass spectrometric analysis of the constituent peptides provides an opportunity to look into proteolytic events such as bioactive peptide processing, membrane protein ectodomain shedding, or even regulated intramembrane proteolysis. If conducted on a large scale, peptidomics has the potential to pinpoint primary cleavage sites. Here a method is described for isolating peptides from cultured cell conditioned media before mass spectrometry analysis.