Pesticide application has little influence on coding and non-coding gene expressions in rice
Pesticide application has little influence on coding and non-coding gene expressions in rice
复制标题
农药施用对水稻编码和非编码基因表达影响不大
DOI：
10.1186/s12864-019-6381-y
复制
发表时间：
2019-12-23
期刊：
影响因子：
4.4
通讯作者：
Wu, Liang
中科院分区：
文献类型：
--
作者：
Muhammad, Sajid;Tan, Jingai;Wu, Liang
背景：农业害虫是作物产量的主要威胁之一。众所周知，施用农药是一种广泛用于消灭害虫的方法，但对环境和生态系统有严重的不良影响；然而，人们并不清楚它是否会影响植物的生理和代谢过程。
结果：在此，我们系统地分析了喷施两种商用农药阿维菌素（ABM）和噻虫嗪（TXM）后水稻的转录组变化。我们发现分别只有有限数量的基因（0.91%）和（1.24%）被ABM和TXM改变，这表明这些农药不会对水稻的性能产生显著影响。然而，我们鉴定出了1140个差异表达基因（DEGs）与105个长链非编码RNA（lncRNAs）相互作用，它们会受到这两种农药的影响，这表明它们在对农药的响应中具有一定的作用。此外，我们检测到274种可变剪接（AS）变化以及宿主基因的表达，阐明了在喷施杀虫剂过程中可变剪接在基因转录控制中的潜在作用。最后，我们鉴定出488个转座子在农药处理后发生了显著变化，导致相邻的编码或非编码转录本发生变化。
结论：总之，我们的研究结果为通过适当的时机和均衡的混合进行害虫管理提供了有价值的见解，在田间药物的可持续施用情况下，这些农药对作物生理没有有害影响。
Background: Agricultural insects are one of the major threats to crop yield. It is a known fact that pesticide application is an extensive approach to eliminate insect pests, and has severe adverse effects on environment and ecosystem; however, there is lack of knowledge whether it could influence the physiology and metabolic processes in plants.Results: Here, we systemically analyzed the transcriptomic changes in rice after a spray of two commercial pesticides, Abamectin (ABM) and Thiamethoxam (TXM). We found only a limited number of genes (0.91%) and (1.24%) were altered by ABM and TXM respectively, indicating that these pesticides cannot dramatically affect the performance of rice. Nevertheless, we characterized 1140 Differentially Expressed Genes (DEGs) interacting with 105 long non-coding RNAs (lncRNAs) that can be impacted by the two pesticides, suggesting their certain involvement in response to farm chemicals. Moreover, we detected 274 alternative splicing (AS) alterations accompanied by host genes expressions, elucidating a potential role of AS in control of gene transcription during insecticide spraying. Finally, we identified 488 transposons that were significantly changed with pesticides treatment, leading to a variation in adjacent coding or non-coding transcripts.Conclusion: Altogether, our results provide valuable insights into pest management through appropriate timing and balanced mixture, these pesticides have no harmful effects on crop physiology over sustainable application of field drugs.