Blue light inhibits the growth of skin tumors in the v-Ha-ras transgenic mouse
Blue light inhibits the growth of skin tumors in the v-Ha-ras transgenic mouse
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DOI：
10.1111/j.1349-7006.2003.tb01420.x
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发表时间：
2003-02-01
期刊：
影响因子：
5.7
通讯作者：
Watanabe, H
中科院分区：
文献类型：
--
作者：
Ohara, M;Kawashima, Y;Watanabe, H
将12-O-十四烷基佛波醇-13-乙酸酯(TPA)涂抹于v-Ha-ras(TG-AC)转基因雌性小鼠背部，每周2次，每次2.5杯，共9周。然后背部皮肤暴露于蓝光(波长470 nm，照度5.7 mW/cm(2))，每天1h，连续9周。应用TPA的小鼠在开始应用后6周出现皮肤肿瘤。用药后6、7、8、9周肿瘤发生率分别为70%、80%、100%和100%，直径1 mm以上的肿瘤个数分别为1、5、10和19个。TPA照射后蓝光照射组小鼠肿瘤发生率分别为10%、40%、60%和80%，肿瘤直径1 mm以上的分别为0、2、5和9个。皮肤组织病理学检查显示，应用TPA在所有10只小鼠中都引起了弥漫性增生、过度角化和乳头状瘤。在4只小鼠中也观察到局限性的表皮增生。TPA照射后蓝光照射组小鼠乳头状瘤发生率较低，角化过度程度较大。过度角化被认为是暴露后的一种倒退变化。这些发现表明，暴露在蓝光下可能是治疗皮肤肿瘤的一种有前途的新方法。(癌症科学2003；94：205-209)。
12-O-Tetradecanoylphorbol-13-acetate (TPA) was applied to the back skin of v-Ha-ras (TG-AC) female transgenic mice at a dose of 2.5 mug/200 mul twice a week for 9 weeks. The back skin was then exposed to blue light (wavelength, 470 nm; irradiance, 5.7 mW/ cm(2)) for 1 h daily for 9 weeks. The mice to which TPA was applied developed skin tumors at 6 weeks after the start of application. The tumor incidence rates at 6, 7, 8 and 9 weeks after the start of application were 70%, 80%, 100% and 100%, respectively, and the numbers of tumors 1 mm or more in diameter were 1, 5, 10 and 19, respectively. In the mice that were exposed to blue light after TPA application, the tumor incidence rates were 10%, 40%, 60% and 80%, respectively, and the numbers of tumors 1 mm or more in diameter were 0, 2, 5 and 9, respectively. Histopathological examination of the skin revealed that TPA application induced diffuse hyperplasia, exaggerated keratinization, and papillomas in all 10 mice. A localized form of epidermal hyperplasia was also observed in 4 mice. The incidence rate of papillomas in the mice that were exposed to blue light after TPA application was lower and the degree of exaggerated keratinization was greater. Exaggerated keratinization was considered to represent a regressive change following exposure. These findings suggest that exposure to blue light may be a promising new approach in the treatment of skin tumors. (Cancer Sci 2003; 94: 205-209).