Human extracellular matrix (ECM) powders for injectable cell delivery and adipose tissue engineering
Human extracellular matrix (ECM) powders for injectable cell delivery and adipose tissue engineering
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DOI：
10.1016/j.jconrel.2009.05.034
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发表时间：
2009-10-01
影响因子：
10.8
通讯作者：
Cho, Yong Woo
中科院分区：
文献类型：
--
作者：
Choi, Ji Suk;Yang, Hyrun-Jin;Cho, Yong Woo
在这里，我们提出了细胞外基质（ECM）粉末来自人类脂肪组织作为可注射的细胞输送载体脂肪组织工程。我们推测，人体脂肪组织可能提供一种理想的生物材料，因为它含有大量的ECM成分，包括胶原蛋白。通过简单的外科手术（抽脂术）获得新鲜的人脂肪组织。除去血液和油成分后，将组织均质化，离心，冷冻干燥，并通过研磨研磨成粉末。在体外研究中，人ECM粉末对于促进三维（3D）细胞培养的细胞附着和增殖是高度有效的。在体内研究中，将含有人脂肪源性干细胞（hASC）的人ECM粉末的悬浮液皮下注射到裸鼠中。注射后8周，观察到大量血管，新形成的组织显示出脂肪形成，细胞内小脂滴积聚。总的来说，移植物显示组织良好的脂肪组织结构，没有任何组织坏死、囊性空间或纤维化的迹象。我们相信，人类ECM粉末可以作为有效的可注射生物材料的组织工程，并有很大的潜力，以满足临床挑战，再生医学，特别是在有关脂肪组织工程。(C)2009爱思唯尔有限公司版权所有。
Here, we present extracellular matrix (ECM) powders derived from human adipose tissue as injectable cell delivery carriers for adipose tissue engineering. We postulate that human adipose tissue may provide an ideal biomaterial because it contains large amounts of ECM components including collagen. Fresh human adipose tissue was obtained by a simple surgical operation (liposuction). After removing blood and oil components, the tissue was homogenized, centrifuged, freeze-dried, and ground to powders by milling. In an in vitro study, the human ECM powders were highly effective for promotion of cell attachment and proliferation for three-dimensional (3D) cell culture. In in vivo studies, suspensions of human ECM powders containing human adipose-derived stem cells (hASCs) were subcutaneously injected into nude mice. At eight weeks post-injection, numerous blood vessels were observed and the newly formed tissue exhibited adipogenesis with accumulated intracellular small lipid droplets. Overall, the grafts showed well-organized adipose tissue constructs without any signs of tissue necrosis, cystic spaces, or fibrosis. We believe that human ECM powders could act as efficient injectable biomaterials for tissue engineering and have great potential for meeting clinical challenges in regenerative medicine, particularly in relation to adipose tissue engineering. (C) 2009 Elsevier B.V. All rights reserved.