Inactivating influenza viruses on surfaces using hydrogen peroxide or triethylene glycol at low vapor concentrations
Inactivating influenza viruses on surfaces using hydrogen peroxide or triethylene glycol at low vapor concentrations
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DOI：
10.1016/j.ajic.2009.06.007
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发表时间：
2009-12-01
影响因子：
4.9
通讯作者：
Spengler, John D.
中科院分区：
文献类型：
--
作者：
Rudnick, Stephen N.;McDevitt, James J.;Spengler, John D.
背景：聚集环境中的表面，如用于大众运输的车辆，可能会被传染性微生物污染，从而促进疾病传播。我们用浓度低于100ppm的过氧化氢(HP)蒸气和含有2ppm三甘醇(TEG)的饱和空气在25℃对受H1N1流感病毒污染的表面进行消毒。方法将流感病毒悬浮液放置在不锈钢悬浮液上，在常温下干燥，然后暴露在长达15分钟至10-90ppm的HP蒸气或TEG饱和空气中。结果：2.5min后，10ppm HP蒸气对病毒灭活效果达99%。在25~29℃的饱和空气中，TEG的消毒速度约为每小时1.3log(10)，比在环境条件下测得的自然灭活速度快约16倍。结论：10ppm HP或2ppm TEG的蒸汽浓度可以提供有效的表面消毒。在这种低浓度下，甚至对飞机的航空电子设备造成损害的可能性预计都将微乎其微。当三甘醇的蒸气浓度为2ppm时，基本上不会对人的健康造成风险。
Background: Surfaces in congregate settings, such as vehicles used for mass transportation, can become contaminated with infectious microorganisms and facilitate disease transmission. We disinfected surfaces contaminated with H1N1 influenza viruses using hydrogen peroxide (HP) vapor at concentrations below 100 ppm and triethylene glycol (TEG)-saturated air containing 2 ppm of TEG at 25 degrees C.Methods Influenza viruses in aqueous suspensions were deposited on stainless-steel coupons, allowed to dry at ambient conditions, and then exposed for up to 15 minutes to 10 to 90 ppm of HP vapor or TEG-saturated air. Virus assays were done on the solution used to wash the viruses from these coupons and from coupons treated similarly but without exposure to HP or TEG vapor.Results: After 2.5 minutes, exposure to 10-ppm HP vapor resulted in 99% inactivation. For air saturated with TEG at 25 to 29 degrees C, the disinfection rate was about 1.3 log(10) reductions per hour, about 16 times faster than the measured natural inactivation rate under ambient conditions.Conclusions: Vapor concentrations of 10 ppm HP or 2 ppm TEG can provide effective surface disinfection. At these low concentrations, the potential for damage to even the avionics of an airplane would be expected to be minimal. At a TEG vapor concentration of 2 ppm, there are essentially no health risks to people.