SEQUENCE AND EXPRESSION OF CAVEOLIN, A PROTEIN-COMPONENT OF CAVEOLAE PLASMA-MEMBRANE DOMAINS PHOSPHORYLATED ON TYROSINE IN ROUS-SARCOMA VIRUS-TRANSFORMED FIBROBLASTS
SEQUENCE AND EXPRESSION OF CAVEOLIN, A PROTEIN-COMPONENT OF CAVEOLAE PLASMA-MEMBRANE DOMAINS PHOSPHORYLATED ON TYROSINE IN ROUS-SARCOMA VIRUS-TRANSFORMED FIBROBLASTS
复制标题
DOI：
10.1073/pnas.89.21.10517
复制
发表时间：
1992-11-01
影响因子：
11.1
通讯作者：
SOPPET, D
中科院分区：
文献类型：
--
作者：
GLENNEY, JR;SOPPET, D
小窝是在内皮和肌肉中丰富的瓶状质膜内陷，但可能存在于所有细胞中。它们含有被认为在其结构和功能中重要的丝状外壳材料。最近的研究表明，一个22 kDa的蛋白质（小窝蛋白）在劳氏肉瘤病毒转化的鸡成纤维细胞的酪氨酸磷酸化是一个组成部分的小窝外套的内部方面的膜。本文报道了鸡小窝蛋白cDNA PCR产物和基因组DNA克隆的蛋白质序列。Caveolin是一种由178个氨基酸组成的独特蛋白质，与GenBank数据库中的其他蛋白质序列相似性很小。疏水性预测表明，一个不寻常的40个氨基酸的疏水区附近的C末端，可用于锚蛋白质的膜。将鸡小窝蛋白在小鼠3T3细胞中表达，免疫荧光显微镜下观察到典型的小窝模式。这包括在许多情况下集中在细胞边缘和阵列中的明亮荧光膜斑块。小窝可能不同于其他膜结构域，至少部分是由于小窝蛋白。
Caveolae are flask-shaped plasma membrane invaginations abundant in endothelium and muscle but may be present in all cells. They contain a filamentous coat material thought to be important in their structure and function. Recent studies have demonstrated that a 22-kDa protein (caveolin) phosphorylated on tyrosine in Rous sarcoma virus-transformed chicken fibroblasts is a component of the caveolae coat on the inner aspect of the membrane. We now report the deduced protein sequence of chicken caveolin derived from cDNA PCR products and genomic DNA clones. Caveolin is a unique protein of 178 amino acids and displays little sequence similarity to other proteins in the GenBank data base. Hydrophobicity predictions indicate an unusual 40-amino acid hydrophobic region near the C terminus that may be used to anchor the protein to the membrane. When chicken caveolin was expressed in mouse 3T3 cells and detected by immunofluorescence microscopy, the typical caveolae pattern was observed. This includes brightly fluorescent membrane patches in many cases concentrated at the margin of cells and in arrays. Caveolae may be distinct from other membrane domains due at least in part to caveolin.