Cognitive impairment in rats induced by nano-CuO and its possible mechanisms
Cognitive impairment in rats induced by nano-CuO and its possible mechanisms
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纳米CuO致大鼠认知障碍及其可能机制
DOI：
10.1016/j.toxlet.2012.07.007
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发表时间：
2012-09-03
影响因子：
3.5
通讯作者：
Zhang, Tao
中科院分区：
文献类型：
--
作者：
An, Lei;Liu, Shichang;Zhang, Tao
一些研究报道了纳米cuo对海马CA1神经元的不良影响，但对纳米cuo在体内的神经毒性知之甚少。在本研究中，我们研究了纳米氧化铜对大鼠空间认知和电生理改变的影响。此外，还测量了大鼠海马的组织学和生化变化。Morris水迷宫（MWM）实验结果显示，纳米cuo处理组大鼠的学习记忆能力明显减弱。长期增强（LTP）实验结果显示，纳米cuo处理组大鼠的场兴奋性突触后电位（fEPSPs）斜率明显低于对照组。纳米cuo处理组海马匀浆中ROS和丙二醛（MDA）水平显著升高，超氧化物歧化酶（SOD）和谷胱甘肽过氧化物酶（GSH-Px）活性显著降低。此外，4-羟基壬烯醛（HNE）和caspase-3的增加暗示了海马细胞凋亡的进展。结果提示，氧化抗氧化稳态受损引起的神经元损伤导致海马LTP受损，与动物行为测试表现不佳有关。2012爱思唯尔爱尔兰有限公司版权所有。
Several studies have reported the adverse effects of nano-CuO on hippocampal CA1 neuron, whereas little has been known about nano-CuO neurotoxicity in vivo. In the present study, we investigated the effects of nano-CuO on spatial cognition and electrophysiological alterations in rats. In addition, histological and biochemical changes in rat's hippocampus were measured as well. Morris water maze (MWM) test showed that learning and memory abilities in nano-CuO-treated group were weakened significantly. The long-term potentiation (LTP) test exhibited that field excitatory postsynaptic potentials (fEPSPs) slopes were significantly lower in nano-CuO-treated group compared to that in control group. Furthermore, the levels of ROS and malonaldehyde (MDA) in hippocampal homogenate of nano-CuO-treated group were considerably enhanced while the activities of superoxide dismutase (SOD) and glutathione peroxidase (GSH-Px) were statistically reduced. Moreover, the enhanced 4-hydroxynonenal (HNE) and caspase-3 implied the progression ofapoptosis in the hippocampus. The results suggested that the neuronal damage, induced by impairing oxidation antioxidation homeostasis, led to the impairment of hippocampal LTP, which was associated with the poor performance of animals in behavior tests. (C) 2012 Elsevier Ireland Ltd. All rights reserved.