Chemical composition and response to dilute-acid pretreatment and enzymatic saccharification of alfalfa, reed canarygrass, and switchgrass
Chemical composition and response to dilute-acid pretreatment and enzymatic saccharification of alfalfa, reed canarygrass, and switchgrass
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DOI：
10.1016/j.biombioe.2006.02.004
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发表时间：
2006-10-01
影响因子：
6
通讯作者：
Sarath, Gautum
中科院分区：
文献类型：
--
作者：
Dien, Bruce S.;Jung, Hans-Joachim G.;Sarath, Gautum
alcohol茎，芦苇金丝雀草，柳枝稷，多年生草本植物物种，有潜力作为生物质能源作物在温带地区，评估其生物转化潜力作为能源作物。每种牧草在两个或三个成熟阶段收获，并分析碳水化合物，木质素，蛋白质，脂质，有机酸和矿物质成分。还评价了用稀硫酸预处理后生物质样品的糖产率，然后使用商业纤维素酶制剂进行酶促糖化。植株总碳水化合物含量为518 ~ 655 gkg（-1）干物质，纤维素含量为209 ~ 322 gkg（-1）干物质。碳水化合物和木质素含量较低的样品从早熟样品相比，样品从晚熟收获。在用稀酸和纤维素酶处理后的糖回收效率方面观察到几个重要趋势。首先，大量的可用碳水化合物是可溶性糖和储存碳水化合物的形式（4.3- 16.3%wt/wt）。稀酸预处理后可溶性糖的回收是有问题的，尤其是果糖。果糖被发现是非常不稳定的稀酸预处理。第二，有效葡萄糖的回收效率与成熟度和木质素含量呈负相关。然而，总葡萄糖产量较高，因为较高的纤维素含量较早成熟的样品相比，后期成熟。最后，发现广泛应用的洗涤剂纤维系统测定的细胞壁多糖是不准确的。洗涤剂纤维法始终高估了纤维素和半纤维素，并大量低估了木质素。爱思唯尔有限公司出版
Alfalfa stems, reed canarygrass, and switchgrass; perennial herbaceous species that have potential as biomass energy crops in temperate regions; were evaluated for their bioconversion potential as energy crops. Each forage species was harvested at two or three maturity stages and analyzed for carbohydrates, lignin, protein, lipid, organic acids, and mineral composition. The biomass samples were also evaluated for sugar yields following pretreatment with dilute sulfuric followed by enzymatic saccharification using a commercial cellulase preparation. Total carbohydrate content of the plants varied from 518 to 655g kg(-1) dry matter (DM) and cellulose concentration from 209 to 322g kg(-1) DM. Carbohydrate and lignin contents were lower for samples from early maturity samples compared to samples from late maturity harvests. Several important trends were observed in regards to the efficiency of sugar recovery following treatments with dilute acid and cellulase. First, a significant amount of the available carbohydrates were in the form of soluble sugars and storage carbohydrates (4.3-16.3% wt/wt). Recovery of soluble sugars following dilute acid pretreatment was problematic, especially that of fructose. Fructose was found to be extremely labile to the dilute acid pretreatments. Second, the efficiency at which available glucose was recovered was inversely correlated to maturity and lignin content. However, total glucose yields were higher for the later maturities because of higher cellulose contents compared to the earlier maturity samples. Finally, cell wall polysaccharides, as determined by the widely applied detergent fiber system were found to be inaccurate. The detergent fiber method consistently overestimated cellulose and hemicellulose and underestimated lignin by substantial amounts. Published by Elsevier Ltd.