STRIKING SIMILARITIES IN AMINO-ACID-SEQUENCE AMONG NONSTRUCTURAL PROTEINS ENCODED BY RNA VIRUSES THAT HAVE DISSIMILAR GENOMIC ORGANIZATION
STRIKING SIMILARITIES IN AMINO-ACID-SEQUENCE AMONG NONSTRUCTURAL PROTEINS ENCODED BY RNA VIRUSES THAT HAVE DISSIMILAR GENOMIC ORGANIZATION
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DOI：
10.1073/pnas.81.14.4358
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发表时间：
1984-01-01
期刊：
影响因子：
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通讯作者：
KAESBERG, P
中科院分区：
文献类型：
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作者：
HASELOFF, J;GOELET, P;KAESBERG, P
植物病毒苜蓿花叶病毒（alfalfa mosaic virus，AMV）和雀麦花叶病毒（brome mosaic virus，BMV）的基因组信息分别由3条RNA组成，而烟草花叶病毒（tobacco mosaic virus，TMV）只有一条RNA。氨基酸序列比较表明，AMV RNA 1和BMV RNA 1以及AMV RNA 2和BMV RNA 2编码的单个蛋白质分别与TMV编码的最大蛋白质的NH 2-末端2/3和COOH-末端1/3相关。在TMV RNA序列中将这2个结构域分开的是琥珀终止密码子，其部分抑制允许下游结构域的翻译。TMV通读结构域和节段植物病毒共有的许多残基也在动物甲病毒辛德毕斯和米德尔堡的非结构多蛋白中发现的通读结构域中保守。尽管在基因组织和表达方面存在很大差异，但所有这些病毒似乎都使用相关蛋白质来实现RNA复制中的共同功能。来自预先存在的病毒或细胞来源的编码和调节序列的功能模块的重配，可能通过RNA重组，可能是RNA病毒进化的重要机制。
The plant viruses alfalfa mosaic virus (AMV) and brome mosaic virus (BMV) each divide their genetic information among 3 RNA while tobacco mosaic virus (TMV) contains a single genomic RNA. Amino acid sequence comparisons suggest that the single proteins encoded by AMV RNA 1 and BMV RNA 1 and by AMV RNA 2 and BMV RNA 2 are related to the NH2-terminal 2/3 and the COOH-terminal 1/3, respectively, of the largest protein encoded by TMV. Separating these 2 domains in the TMV RNA sequence is an amber termination codon, whose partial suppression allows translation of the downstream domain. Many of the residues that the TMV read-through domain and the segmented plant viruses have in common are also conserved in a read-through domain found in the nonstructural polyprotein of the animal alphaviruses Sindbis and Middelburg. Despite substantial differences in gene organization and expression, all of these viruses appear to use related proteins for common functions in RNA replication. Reassortment of functional modules of coding and regulatory sequence from preexisting viral or cellular sources, perhaps via RNA recombination, may be an important mechanism in RNA virus evolution.