Age-related changes in the murine cochlear lateral wall
Age-related changes in the murine cochlear lateral wall
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DOI：
10.1016/s0378-5955(99)00170-7
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发表时间：
2000-01-01
期刊：
影响因子：
2.8
通讯作者：
Mogi, G
中科院分区：
文献类型：
--
作者：
Ichimiya, I;Suzuki, M;Mogi, G
采用电生理和组织化学方法对C57BL/6小鼠耳蜗进行观察，探讨老年性耳聋的病理变化。6周龄小鼠的平均听觉脑干反应阈值显著低于6月龄小鼠和1岁小鼠。6月龄后组织学观察发现耳蜗有改变。常规苏木精和伊红（H&E）染色显示Corti器官组织紊乱，螺旋神经节细胞数量减少，血管纹萎缩。虽然H&E染色和II型胶原免疫标记在螺旋韧带（SL）未见明显变化，但该区域连接蛋白26染色密度降低。钠-钾-腺苷三磷酸酶免疫标记在St中增加，而其平均密度在血管纹中没有显著改变。这些结果表明，随着年龄的增长，下皮层可能是导致耳蜗功能障碍的区域之一。(C) 2000 Elsevier Science B.V.版权所有
Cochleas From C57BL/6 mice were investigated electrophysiologically and histochemically to evaluate the pathology of presbycusis. The average auditory brainstem response thresholds from 6-week-old mice were significantly lower than those of 6-month-old mice and those of I-year-old mice. Histologic observation revealed changes in the cochlea after age 6 months. Conventional hematoxylin and eosin (H&E) staining showed disorganization of the organ of Corti, a decrease in the number of spiral ganglion cells, and atrophy of the stria vascularis. Although H&E staining and type II collagen immunolabeling did not show obvious changes in the spiral ligament (SL), the density of connexin 26 staining was reduced in this region. Sodium-potassium-adenosinetriphosphatase immunolabeling was increased in the St., whereas its average density was not significantly altered in the stria vascularis. These results suggest that the SL could be among the regions responsible for cochlear malfunction with aging. (C) 2000 Elsevier Science B.V. All rights reserved.