Inhibitory effects of basic or neutral phospholipid on acidic phospholipid-mediated dissociation of adenine nucleotide bound to DnaA protein, the initiator of chromosomal DNA replication
Inhibitory effects of basic or neutral phospholipid on acidic phospholipid-mediated dissociation of adenine nucleotide bound to DnaA protein, the initiator of chromosomal DNA replication
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DOI：
10.1074/jbc.m212202200
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发表时间：
2003-08-01
影响因子：
4.8
通讯作者：
Sekimizu, K
中科院分区：
文献类型：
--
作者：
Ichihashi, N;Kurokawa, K;Sekimizu, K
DnaA蛋白是细菌染色体DNA复制的启动子，其活性受酸性磷脂如磷脂酰甘油（PG）或心磷脂（CL）的调节，通过促进结合的腺嘌呤核苷酸的交换反应。从指数生长的金黄色葡萄球菌细胞中分离的总脂质促进了与S.金黄色葡萄球菌DnaA蛋白，而来自稳定期细胞的蛋白是惰性的。从静止期细胞的总脂质的馏分显示，基本磷脂，赖氨酰磷脂酰甘油（LPG），抑制PG-或CL-促进从DnaA蛋白释放ATP。在稳定期期间LPG浓度增加。一个整合缺失mprF突变体，其中液化石油气丢失的静止期细胞的总脂质的一部分，促进了ATP从DnaA蛋白的释放。两性离子磷脂，磷脂酰乙醇胺，也抑制PG促进ATP的释放。这些结果表明，DnaA蛋白与酸性磷脂的相互作用可能受到调节的变化，在不同的生长阶段的细胞膜的磷脂组成。此外，mprF突变体在体内表现出每个细胞的起源量增加，表明LPG参与调节细胞周期事件。
DnaA protein activity, the initiator of chromosomal DNA replication in bacteria, is regulated by acidic phospholipids such as phosphatidylglycerol ( PG) or cardiolipin ( CL) via facilitation of the exchange reaction of bound adenine nucleotide. Total lipid isolated from exponentially growing Staphylococcus aureus cells facilitated the release of ATP bound to S. aureus DnaA protein, whereas that from stationary phase cells was inert. Fractionation of total lipid from stationary phase cells revealed that the basic phospholipid, lysylphosphatidylglycerol (LPG), inhibited PG- or CL-facilitated release of ATP from DnaA protein. There was an increase in LPG concentration during the stationary phase. A fraction of the total lipid from stationary phase cells of an integrational deletion mprF mutant, in which LPG was lost, facilitated the release of ATP from DnaA protein. A zwitterionic phospholipid, phosphatidylethanolamine, also inhibited PG- facilitated ATP release. These results indicate that interaction of DnaA protein with acidic phospholipids might be regulated by changes in the phospholipid composition of the cell membrane at different growth stages. In addition, the mprF mutant exhibited an increased amount of origin per cell in vivo, suggesting that LPG is involved in regulating the cell cycle event(s).