Characterization and gene expression analysis of novel matched primary and metastatic renal cell carcinoma cell lines
Characterization and gene expression analysis of novel matched primary and metastatic renal cell carcinoma cell lines
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DOI：
10.3892/or_00000034
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发表时间：
2008-09-01
期刊：
影响因子：
4.2
通讯作者：
Nishimura, Toshihide
中科院分区：
文献类型：
--
作者：
Ohno, Yoshio;Izumi, Miki;Nishimura, Toshihide
尽管分子生物学的最新进展已经阐明了几种癌症转移的机制，但肾细胞癌（RCC）转移的分子机制仍不清楚。从同一肾细胞癌患者匹配的原发肿瘤和肾上腺转移瘤的手术标本中成功建立了两个肾细胞癌细胞系，分别命名为TMK-1P和TMK-1M。使用各种方法完成了广泛的表征，包括基质胶侵袭测定、DNA 微阵列分析和实时逆转录酶 (RT)-聚合酶链式反应 (PCR)。虽然TMK-1P的生长速度比TMK-1M快，但TMK-1M的侵袭能力高于TMK-1P。 DNA微阵列分析显示，TMK-1M中与细胞粘附和细胞外基质分子相关的基因表达存在较大差异，其中hexabrachion（tenascin-C）、表皮生长因子受体、钙粘蛋白-6和β1-catenin下调，而67 kDa层粘连蛋白受体I和转化生长因子-β诱导的68 kDa蛋白（β ig-h3）上调。实时 RT-PCR 分析证实了两种细胞系之间的基因表达差异。 RCC 细胞系可用于研究肿瘤侵袭和筛选转移标志物。
Despite recent advances in molecular biology that have clarified the mechanisms involved in the metastasis of several types of cancer, the molecular mechanism underlying the metastasis of renal cell carcinoma (RCC) remains unclear. Two RCC cell lines were successfully established from the surgical specimens of a matched primary tumor and adrenal metastasis from the same RCC patient, and were designated as TMK-1P and TMK-1M, respectively. Extensive characterization was accomplished using various methods, including the Matrigel invasion assay, DNA microarray analysis and real-time reverse transcriptase (RT)-polymerase chain reaction (PCR). While TMK-1P grew faster than TMK-1M, the invasive ability of TMK-1M was higher than that of TMK-1P. DNA microarray analysis showed a large differential expression of genes related to cell adhesion and the extracellular matrix molecules of which hexabrachion (tenascin-C), epidermal growth factor receptor, cadherin-6, and beta 1-catenin were down-regulated, and the 67 kDa laminin receptor I and transforming growth factor-beta-induced 68 kDa protein (beta ig-h3) were up-regulated in TMK-1M. Real-time RT-PCR analysis confirmed this differential gene expression between the two cell lines. The RCC cell lines may be useful in studying tumor invasion and screening markers for metastasis.