A Simple Nanoscale Interface Directs Alignment of a Confluent Cell Layer on Oxide and Polymer Surfaces.
A Simple Nanoscale Interface Directs Alignment of a Confluent Cell Layer on Oxide and Polymer Surfaces.
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简单的纳米级界面引导氧化物和聚合物表面上的汇合细胞层对齐。
DOI：
10.1039/c3tb20565g
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发表时间：
2013
期刊：
影响因子：
--
通讯作者：
Schwarzbauer,JeanE
中科院分区：
文献类型：
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作者：
Donnelly,PatrickE;Jones,CaseyM;Bandini,StephenB;Singh,Shivani;Schwartz,Jeffrey;Schwarzbauer,JeanE
描述了细胞铺展和增殖的模板化，其产生在整个二维表面上对齐的细胞汇合层。该模板是一种反应性的双组分界面，在硅和几种生物材料聚合物上以纳米厚度、微米级图案分三步合成。在该方法中，首先在减压下将挥发性锆醇盐络合物沉积到通过光刻法制备的表面图案上;然后加热基底以使有机配体热固化以形成表面结合的氧化锆图案。该氧化物层的厚度范围为10至70纳米，其由醇盐络合物沉积时间控制。用1，4-丁烷二膦酸处理氧化物层，得到单层图案，其组成和与光刻掩模的空间一致性通过光谱确定。NIH 3 T3成纤维细胞和人骨髓来源的间充质干细胞附着和扩散与图案对齐，而不受物理手段或亲细胞和疏细胞分子阵列的约束。随着细胞生长以在整个基底表面上形成汇合的单层，保持细胞与图案对齐。
Templating of cell spreading and proliferation is described that yields confluent layers of cells aligned across an entire two-dimensional surface. The template is a reactive, two-component interface that is synthesized in three steps in nanometer thick, micron-scaled patterns on silicon and on several biomaterial polymers. In this method, a volatile zirconium alkoxide complex is first deposited at reduced pressure onto a surface pattern that is prepared by photolithography; the substrate is then heated to thermolyze the organic ligands to form surface-bound zirconium oxide patterns. The thickness of this oxide layer ranges from 10 to 70 nanometers, which is controlled by alkoxide complex deposition time. The oxide layer is treated with 1,4-butanediphosphonic acid to give a monolayer pattern whose composition and spatial conformity to the photolithographic mask are determined spectroscopically. NIH 3T3 fibroblasts and human bone marrow-derived mesenchymal stem cells attach and spread in alignment with the pattern without constraint by physical means or by arrays of cytophilic and cytophobic molecules. Cell alignment with the pattern is maintained as cells grow to form a confluent monolayer across the entire substrate surface.