Determination of carbon-centered radicals in aqueous solution by liquid chromatography with fluorescence detection
Determination of carbon-centered radicals in aqueous solution by liquid chromatography with fluorescence detection
复制标题
液相色谱荧光检测法测定水溶液中的碳中心自由基
DOI：
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发表时间：
1990
期刊：
影响因子：
--
通讯作者：
N. Blough
中科院分区：
文献类型：
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作者：
D. Kieber;N. Blough
一个简单的方法来检测subnanomolar到微摩尔水平的光化学产生的碳为中心的自由基在水溶液中已经开发和优化。该方法是基于水溶性氨基氮氧化物的自由基的有效捕获，然后用荧光胺衍生化捕获的产物以产生高荧光加合物。这些加合物可以通过反相高效液相色谱法分离并通过荧光法检测。荧光衍生物在数天内是稳定的。主要由试剂干扰确定的检测限范围为0.3至1 nM/分析物，500 μ L进样，信噪比为2。在1-10 nM范围内测定加合物浓度的方法精密度为2.4 - 8.4%相对标准偏差（n = 6）。与电子顺磁共振谱/自旋捕获的直接比较说明了我们的技术的优势。该方法的一个重要特点是，它允许同时检测一系列的自由基，通过研究各种天然水样和苏瓦尼河富里酸中的自由基的光化学生产证明。
A simple method to detect subnanomolar to micromolar levels of photochemically generated carbon-centered radicals in aqueous solutions has been developed and optimized. This method is based on the efficient trapping of radicals by a water-soluble amino nitroxide, followed by derivatization of the trapped products with fluorescamine to produce highly fluorescent adducts. These adducts can be separated by reversed-phase high-performance liquid chromatography and detected fluorometrically. The fluorescent derivatives are stable over a period of days. The detection limit, primarily determined by reagent interferences, ranged from 0.3 to 1 nM per analyte for a 500-{mu}L injection at a signal-to-noise ratio of two. The precision of the method for the determination of adduct concentrations in the 1-10 nM range varied from 2.4 to 8.4% relative standard deviation (n = 6). A direct comparison with electron paramagnetic resonance spectroscopy/spin trapping illustrates the advantages of our technique. One important feature of the method is that it permits the simultaneous detection of an array of radicals, as demonstrated through the study of the photochemical production of radicals in a variety of natural water samples and in Suwanee River fulvic acid.
.OH 和 .O2 的 DMPO 自旋加合物的细胞诱导衰变。
影响因子：
7.4
作者：
Samuni,A;Samuni,A;Swartz,HM
通讯作者：
Swartz,HM