DNA synthesis is not necessary for osteoclastic responses to parathyroid hormone in cultured fetal rat long bones.
DNA synthesis is not necessary for osteoclastic responses to parathyroid hormone in cultured fetal rat long bones.
复制标题
在培养的胎鼠长骨中，DNA 合成对于破骨细胞对甲状旁腺激素的反应不是必需的。
DOI：
10.1172/jci111156
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发表时间：
1983
期刊：
影响因子：
--
通讯作者：
Hock,JM
中科院分区：
文献类型：
--
作者：
Lorenzo,JA;Raisz,LG;Hock,JM
Osteoclasts, the principal cells mediating bone resorption, are believed to increase their size, number, and resorbing activity in response to parathyroid hormone (PTH) through mechanisms dependent upon the fusion of specific mononuclear precursor cells into either new or existing multinucleated osteoclasts. To address the question of whether these actions of PTH are dependent on the replication of osteoclast precursor cells, we examined the ability of an inhibitor of DNA synthesis, hydroxyurea (HU), to alter bone resorption, osteoclast formation, and DNA synthesis in cultured fetal rat bones treated with PTH. We found that HU significantly reduced [3H]thymidine incorporation into the bones and labeling of osteoclast nuclei by greater than 90%, but did not prevent PTH from stimulating bone resorption, measured as the release of 45Ca, or from increasing the number of osteoclasts in the bones. In bones cultured without PTH, HU decreased the rate of bone resorption, but not the number of osteoclasts per bone. We conclude that in fetal rat bone cultures, PTH can increase osteoclast number and stimulate bone resorption by affecting existing osteoclasts and osteoclast precursors, and that replication of osteoclast precursor cells is not necessary for PTH to stimulate a resorptive response. In unstimulated cultures it appears that HU inhibits bone resorption by affecting mechanisms that are independent of changes in osteoclast number and that may be influenced by cell replication or other unknown factors.
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骨器官培养
DOI：
--
发表时间：
1979
期刊：
影响因子：
--
作者：
P. Stern;L. Raisz
通讯作者：
L. Raisz
骨细胞与破骨细胞的发育关系：利用鹌鹑鸡核标记物进行软骨内骨化的研究☆
DOI：
--
发表时间：
1978
期刊：
影响因子：
--
作者：
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N. L. Le Douarin
甲状旁腺提取物对体内细胞活性和血浆钙水平的影响。
影响因子：
4
作者：
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通讯作者：
M. Owen
甲状旁腺激素对骨重塑和钙稳态影响的研究。
影响因子：
4.2
作者：
R. Talmage
通讯作者：
R. Talmage
[99] 戊糖分析测定组织中的核酸
DOI：
--
发表时间：
1957
期刊：
影响因子：
--
作者：
W. C. Schneider
通讯作者：
W. C. Schneider