An HPLC method for the quantification of sterols in edible seaweeds
An HPLC method for the quantification of sterols in edible seaweeds
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DOI：
10.1002/bmc.316
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发表时间：
2004-04-01
影响因子：
1.8
通讯作者：
López-Cervantes, J
中科院分区：
文献类型：
--
作者：
Sánchez-Machado, DI;López-Hernández, J;López-Cervantes, J
建立了一种高效液相色谱法测定食用海藻中甾醇含量的方法。在APCI阳性模式下，采用高效液相色谱/质谱法（HPLC- ms）对甾醇进行鉴定。样品用1 m乙醇KOH回流皂化，不可皂化部分用己烷萃取。采用高效液相色谱-紫外检测（HPLCUV），色谱柱为15 × 0.4 cm Kromasil 100 C-18 5 mum（流动相：70 v/v甲醇：乙腈，流速1.2 mL/min，柱温30℃，检测波长205 nm）。方法focus甾醇重复性好（变异系数2.4%）。测定了罐装或干燥褐海带（Himanthalia elongata, Undaria pinnatifida, Laminaria ochroleuca）和红海带（Palmaria sp., Porphyra sp.）的甾醇含量。棕色海藻的主要甾醇为焦脂醇（占总甾醇含量的83 ~ 97%，662 ~ 2320马克杯/g干重），红色海藻的主要甾醇为去氨醇（占总甾醇含量的87 ~ 93%，187 ~ 337马克杯/g干重）。版权所有：John Wiley Sons， Ltd. 2004
This study presents an HPLC method for the quantification of sterols in edible seaweeds. Sterols were identified by HPLC/mass spectrometry (HPLC-MS) in positive APCI mode. The samples were saponified by refluxing with 1 m ethanolic KOH, and the non-saponifiable fraction was extracted with hexane. Sterols were quantified by HPLC with UV detection (HPLCUV), on a 15 x 0.4 cm Kromasil 100 C-18 5 mum column (mobile phase 30:70 v/v methanol:acetonitrile; flow rate 1.2 mL/min; column temperature 30degreesC; detection wavelength 205 nm). Method repeatability for fucosterol was good (coefficient of variation 2.4%). Sterol contents were determined in canned or dried brown seaweeds (Himanthalia elongata, Undaria pinnatifida, Laminaria ochroleuca) and red seaweeds (Palmaria sp., Porphyra sp.). The predominant sterol was fucosterol in brown seaweeds (83-97% of total sterol content; 662-2320 mug/g dry weight), and desmosterol in red seaweeds (87-93% of total sterol content; 187-337 mug/g dry weight). Copyright (C) 2004 John Wiley Sons, Ltd.