REGULATION OF MOUSE OOCYTE MATURATION - EFFECT OF ELEVATING CUMULUS CELL CAMP ON OOCYTE CAMP LEVELS
REGULATION OF MOUSE OOCYTE MATURATION - EFFECT OF ELEVATING CUMULUS CELL CAMP ON OOCYTE CAMP LEVELS
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DOI：
10.1095/biolreprod33.3.698
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发表时间：
1985-01-01
影响因子：
3.6
通讯作者：
SCHULTZ, RM
中科院分区：
文献类型：
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作者：
BORNSLAEGER, EA;SCHULTZ, RM
我们重新研究了卵丘细胞cAMP通过连接两种细胞类型的缝隙连接进入卵母细胞的可能性(Shultz等人，1983a)。由于我们最近的结果表明小鼠卵母细胞具有非常活跃的环核苷酸磷酸二酯酶(PDE)(Bornsleger等人，1984)，我们修改了我们的实验方案，以确保在整个实验过程中小鼠卵母细胞的PDE活性被抑制。我们的结果证明了cAMP从卵丘细胞到卵母细胞的明显转移；这些结果根据目前哺乳动物卵母细胞成熟调控模型进行了讨论。
We have reexamined the possibility that cumulus cell cAMP can enter the oocyte via the gap junctions connecting the two cell types (Shultz et al., 1983a). Since our recent results indicate that the mouse oocyte possess a very active cyclic nucleotide phosphodiesterase (PDE) (Bornslaeger et al., 1984), we have altered our experimental protocol to ensure that mouse oocyte PDE activity is inhibited throughout the duration of an experiment. Our results demonstrate the apparent transfer of cAMP from cumulus cells to the oocyte; these results are discussed in terms of current models for regulation of mammalian oocyte maturation.