Genome-wide association analysis and differential expression analysis of resistance to Sclerotinia stem rot in Brassica napus
Genome-wide association analysis and differential expression analysis of resistance to Sclerotinia stem rot in Brassica napus
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甘蓝型油菜抗核病菌茎腐病的全基因组关联分析及差异表达分析
DOI：
10.1111/pbi.12501
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发表时间：
2016
影响因子：
13.8
通讯作者：
Li Jiana
中科院分区：
文献类型：
--
作者：
Wei Lijuan;Jian Hongju;Lu Kun;Filardo Fiona;Yin Nengwen;Liu Liezhao;Qu Cunmin;Li Wei;Du Hai;Li Jiana
甘蓝型油菜是世界上最重要的油料作物之一，由真菌核盘菌引起的茎腐病会导致产量和质量的重大损失。为了阐明抗性基因和发病机制相关基因，使用 Illumina 60KBrassicaSNP（单核苷酸多态性）阵列对 347 个材料进行了全基因组关联分析。此外，采用离体茎接种法筛选出5个高抗性(R)和易感(S)B。 油菜，接种后 48 小时。 菌核用于转录组测序。我们在 A8 和 C6 染色体上发现了 17 个与茎抗性相关的显着关联，其中 5 个位于 A8 上，12 个位于 C6 上。 A8 上鉴定的 SNP 位于 409 kb 单倍型块中，C6 上的 SNP 与之前的 QTL 定位工作一致。转录组分析表明 S. 菌核感染会激活免疫系统、硫代谢，尤其是 R 和 S 基因型的谷胱甘肽 (GSH) 和芥子油苷。发现与茉莉酸途径、木质素生物合成、防御反应、信号转导和编码转录因子相关的 R 基因型特有的基因。 SNP 性状关联和转录组测序分析均鉴定出 24 个基因，其中包括 tau 类谷胱甘肽 S 转移酶 (GSTU) 基因簇。这项研究为了解植物对硫的反应的分子机制提供了有用的见解。 菌核。
Brassica napusis one of the most important oil crops in the world, and stem rot caused by the fungusSclerotinia sclerotiorumresults in major losses in yield and quality. To elucidate resistance genes and pathogenesis‐related genes, genome‐wide association analysis of 347 accessions was performed using the Illumina 60KBrassicaSNP (single nucleotide polymorphism) array. In addition, the detached stem inoculation assay was used to select five highly resistant (R) and susceptible (S)B. napuslines, 48 h postinoculation withS. sclerotiorumfor transcriptome sequencing. We identified 17 significant associations for stem resistance on chromosomes A8 and C6, five of which were on A8 and 12 on C6. The SNPs identified on A8 were located in a 409‐kb haplotype block, and those on C6 were consistent with previous QTL mapping efforts. Transcriptome analysis suggested thatS. sclerotioruminfection activates the immune system, sulphur metabolism, especially glutathione (GSH) and glucosinolates in both R and S genotypes. Genes found to be specific to the R genotype related to the jasmonic acid pathway, lignin biosynthesis, defence response, signal transduction and encoding transcription factors. Twenty‐four genes were identified in both the SNP‐trait association and transcriptome sequencing analyses, including a tau class glutathione S‐transferase (GSTU) gene cluster. This study provides useful insight into the molecular mechanisms underlying the plant's response toS. sclerotiorum.