Metal Carbonyls for the Biointerference-Free Ratiometric Surface-Enhanced Raman Spectroscopy-Based Assay for Cell-Free Circulating DNA of Epstein-Barr Virus in Blood
Metal Carbonyls for the Biointerference-Free Ratiometric Surface-Enhanced Raman Spectroscopy-Based Assay for Cell-Free Circulating DNA of Epstein-Barr Virus in Blood
复制标题
金属羰基用于基于无生物干扰的比例表面增强拉曼光谱测定血液中 Epstein-Barr 病毒的无细胞循环 DNA
DOI：
10.1021/acs.analchem.8b01931
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发表时间：
2018-06-19
影响因子：
7.4
通讯作者：
Kong, Kien Voon
中科院分区：
文献类型：
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作者：
Lin, Duo;Gong, Tianxun;Kong, Kien Voon
利用金属羰基化合物的光谱特性，设计了一种检测鼻咽癌患者血液中EB病毒游离循环DNA(CfDNA)的表面增强拉曼光谱(SERS)比色法。该方法包括作为DNA探针的羰基Re(Re-CO)，嵌入在SERS活性底物中的作为内参照物的Os-CO(Os-CO)，以及底物表面的链霉亲和素。CfDNA与生物素化捕获序列的杂交导致cfDNA固定在底物上。Re-CO通过柔红霉素(DNR)与cfDNA结合时，在2113 cm(-1)处出现一个强对称性伸缩振动峰，与Os-CO(2025 cm(-1))有光谱重叠。这导致I-2113/I-2025比率增加，并与cfDNA定量相关。由于I-2113/I-2025的强度比位于生物分子的SERS谱的无声区域(1780-2200 cm(-1))，这种SERS方法可以很容易地用于检测患者血液样本中的cfDNA。
By taking advantage of the spectral properties of metal carbonyls, we have designed a surface-enhanced Raman spectroscopy (SERS) ratiometric assay for measuring cellfree circulating DNA (cfDNA) from Epstein-Barr virus in blood for nasopharyngeal carcinoma (NPC). This assay consists of a rhenium carbonyl (Re-CO) to serve as a DNA probe, an osmium carbonyl (Os-CO) embedded within the SERS-active substrate as an internal reference, and a streptavidin layer on the surface of the substrate. Hybridization of cfDNA with biotinylated-capture sequence leads to immobilization of cfDNA on the substrate. The binding of Re-CO via daunorubicin (DNR) to cfDNA is accompanied by an appearance of a strong symmetry stretching vibrations peak at 2113 cm(-1), which has spectral overlap with Os-CO (2025 cm(-1)). This results in an increase in the I-2113/I-2025 ratio and quantitatively correlates with cfDNA. This SERS assay can be readily used to detect cfDNA in blood samples from patients due to the intensity ratio of I-2113/I-2025 lying in a silent region (1780-2200 cm(-1)) in the SERS spectrum of the biomolecules.