Secretion of the Caulobacter crescentus S-layer protein:: Further localization of the C-terminal secretion signal and its use for secretion of recombinant proteins
Secretion of the Caulobacter crescentus S-layer protein:: Further localization of the C-terminal secretion signal and its use for secretion of recombinant proteins
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DOI：
10.1128/jb.182.11.3298-3301.2000
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发表时间：
2000-06-01
影响因子：
3.2
通讯作者：
Smit, J
中科院分区：
文献类型：
--
作者：
Bingle, WH;Nomellini, JF;Smit, J
新月柄杆菌S层蛋白（RsaA）的分泌信号定位于分子的C-末端82个氨基酸。蛋白质产率研究表明，RsaA的336或242个C-末端残基介导了每升培养液中>50 mg纤维素酶过客蛋白的分泌。
The secretion signal of the Caulobacter crescentus S-layer protein (RsaA) was localized to the C-terminal 82 amino acids of the molecule. Protein yield studies showed that 336 or 242 C-terminal residues of RsaA mediated secretion of >50 mg of a cellulase passenger protein per liter to the culture fluids.