Cellulose-water interactions during enzymatic hydrolysis as studied by time domain NMR
Cellulose-water interactions during enzymatic hydrolysis as studied by time domain NMR
复制标题
DOI：
10.1007/s10570-008-9222-8
复制
发表时间：
2008-10-01
期刊：
影响因子：
5.7
通讯作者：
Elder, Thomas
中科院分区：
文献类型：
--
作者：
Felby, Claus;Thygesen, Lisbeth G.;Elder, Thomas
利用时间域低场核磁共振可以研究水在纤维素基质中的不同状态和位置。在这项工作中，我们展示了酶解如何影响滤纸纤维中与纤维素相关的水的状态和位置。滤纸中的水有三个位置：(1)与微原纤维表面有关的结合水，(2)细胞壁或纤维基质中的水，(3)管腔和纤维之间的毛细管水。纤维素酶对纤维素-水相互作用的不同作用机制可以从纤维素酶对纤维素-水相互作用的影响中看出，而内切酶和外切酶之间的协同作用可以很容易地通过时间域核磁共振检测出来。一个有趣的观察是，可以将纤维素纤维中水的状态和位置与酶水解时的结构变化联系起来。
The different states and locations of water within the cellulose matrix can be studied by the use of time domain low field NMR. In this work we show how the state and location of water associated with cellulose in filter paper fibers are affected by enzymatic hydrolysis. Three locations of water were identified in the filter paper; (1) bound water associated with the microfibril surfaces and (2) water in the cell wall or cellulose matrix and (3) capillary water in the lumens and between fibers. The different mechanisms of cellulase enzymes can be seen in their effect on the cellulose-water interactions and the synergistic effects between endo- and exo enzymes can be easily detected by time domain NMR. An interesting observation is that it is possible to link the state and location of water within the cellulose fiber with structural changes upon enzymatic hydrolysis.