Characterization of rabbit myocardial phospholipids with 31P nuclear magnetic resonance.
Characterization of rabbit myocardial phospholipids with 31P nuclear magnetic resonance.
复制标题
用 31P 核磁共振表征兔心肌磷脂。
DOI：
10.1016/0005-2760(80)90117-4
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发表时间：
1980
期刊：
影响因子：
--
通讯作者：
Sobel,BE
中科院分区：
文献类型：
--
作者：
Mogelson,S;WilsonJr,GE;Sobel,BE
为了减少磷脂在提取和分析过程中的化学修饰，我们开发并评估了一种用31 P NMR分析组织磷脂的方法。结果表明：（1）氯仿/甲醇组织提取液的~（31）P NMR是一种简便、快速的心肌磷脂表征技术。(2)正常兔心肌含有很少的，如果有的话，溶血甘油磷脂。(3)磷脂的常规提取和色谱法可通过酸催化水解将胆碱和乙醇胺缩醛磷脂转化为溶血甘油磷脂。
To reduce chemical modifications of phospholipids during extraction and analysis, we developed and evaluated a method for analyzing tissue phospholipids with 31P NMR. Our results indicate:(1) 31P NMR of chloroform/methanol tissue extracts is a convenient and rapid technique for characterizing myocardial phospholipids.(2) Normal rabbit myocardium contains little, if any, lysoglycerophospholipid.(3) Conventional extraction and chromatography of phospholipids may convert choline and ethanolamine plasmalogens to lysoglycerophospholipids by acid-catalyzed hydrolysis.